Section of Gynecologic Oncology, Department of Obstetrics and Gynecology, China Medical University, 91 Hsueh Shih Road, Taichung 404, Taiwan.
J Exp Biol. 2010 Jun 15;213(Pt 12):2079-85. doi: 10.1242/jeb.039768.
The current study investigated the possible effects of static magnetic fields (SMFs) on the developmental and aging processes of Caenorhabditis elegans. Nematodes were grown in the presence of SMFs of strengths varying from 0 to 200 mT. The rate of development and the lifespan were recorded. Treatment with a 200 mT SMF reduced the development time from the L2 to the L3 stage by 20%, from L3 to L4 by 23%, and from L4 to young adult by 31%. After SMF treatment, the average lifespan was reduced from 31 days to 24 days for wild-type nematodes. The up-regulation of clk-1, lim-7, daf-2, unc-3 and age-1 after SMF treatment was verified by quantitative real-time RT-PCR. Apparently, induction of gene expression is selective and dose dependent. The total developmental time was significantly reduced for the lin-4, lin-14, lin-41 and lim-7 mutants, but not for the let-7, clk-1, unc-3 and age-1 mutants. Lifespan analyses revealed that the let-7, unc-3 and age-1 mutants were not affected by SMF treatment. Here we show that SMFs accelerate nematode development and shorten nematode lifespan through pathways associated with let-7, clk-1, unc-3 and age-1.
本研究探讨了静磁场（SMFs）对秀丽隐杆线虫发育和衰老过程的可能影响。线虫在 0 至 200 mT 强度的 SMFs 存在下生长。记录发育速度和寿命。用 200 mT 的 SMF 处理将从 L2 到 L3 阶段的发育时间缩短了 20%，从 L3 到 L4 缩短了 23%，从 L4 到幼年期缩短了 31%。SMF 处理后，野生型线虫的平均寿命从 31 天缩短到 24 天。通过定量实时 RT-PCR 验证了 SMF 处理后 clk-1、lim-7、daf-2、unc-3 和 age-1 的上调。显然，基因表达的诱导是选择性的和剂量依赖性的。lin-4、lin-14、lin-41 和 lim-7 突变体的总发育时间明显缩短，但 let-7、clk-1、unc-3 和 age-1 突变体则没有。寿命分析表明，let-7、unc-3 和 age-1 突变体不受 SMF 处理的影响。我们在此表明，SMFs 通过与 let-7、clk-1、unc-3 和 age-1 相关的途径加速线虫发育并缩短线虫寿命。
