棘白菌素类、嘧啶类和多烯类药物对白色念珠菌生物膜的抗药性中 Fks1p 和基质葡聚糖的作用
Role of Fks1p and matrix glucan in Candida albicans biofilm resistance to an echinocandin, pyrimidine, and polyene.
机构信息
Department of Medicine, University of Wisconsin, 600 Highland Ave., H4/572 Clinical Sciences Center, Madison, WI 53792, USA.
出版信息
Antimicrob Agents Chemother. 2010 Aug;54(8):3505-8. doi: 10.1128/AAC.00227-10. Epub 2010 Jun 1.
Abstract
Candida infections frequently involve drug-resistant biofilm growth on device surfaces. Glucan synthase gene FKS1 has been linked to triazole resistance in Candida biofilms. We tested the impact of FKS1 modulation on susceptibility to additional antifungal classes. Reduction of FKS1 expression rendered biofilms more susceptible to amphotericin B, anidulafungin, and flucytosine. Increased resistance to anidulafungin and amphotericin B was observed for biofilms overexpressing FKS1. These findings suggest that Candida biofilm glucan sequestration is a multidrug resistance mechanism.
摘要
念珠菌感染常涉及在器械表面形成耐药生物膜。葡聚糖合酶基因 FKS1 与念珠菌生物膜中的唑类耐药性有关。我们测试了 FKS1 调节对其他抗真菌类药物敏感性的影响。FKS1 表达的减少使生物膜对两性霉素 B、阿尼鲁单抗和氟胞嘧啶更敏感。过表达 FKS1 的生物膜对阿尼鲁单抗和两性霉素 B 的耐药性增加。这些发现表明，念珠菌生物膜葡聚糖隔离是一种多药耐药机制。
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