University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030-1605, USA.
J Dent Res. 2010 Sep;89(9):921-6. doi: 10.1177/0022034510369460. Epub 2010 Jun 2.
Lactobacillus paracasei DSMZ16671, even if heat-killed, sensitively co-aggregates mutans streptococci specifically. Mutans streptococci are strongly implicated in caries induction in humans and rodents. We hypothesized: (1) that S. mutans recoveries from rats' teeth in vivo will decrease, with an associated decrease in caries, if these lactobacilli are fed to rats in an established caries model; and (2) that toxicological assays of these lactobacilli will show them to be benign. Four groups of specific-pathogen-free rats were formed: S. mutans 10449S-inoculated/16671-supplemented diet; un-inoculated/16671-supplemented diet; S. mutans 10449S-inoculated/placebo diet; and un-inoculated/placebo diet. Standard tests of toxicity and mutagenicity of heat-killed DSMZ16671 were performed. S. mutans recoveries were significantly reduced both in mid-experiment and at termination, as were caries lesion scores for the rats inoculated by S. mutans and fed the DSMZ16671 supplement, by comparison with controls. Neither toxicity nor mutagenicity of DSMZ16671 was detected. Use of heat-killed DSMZ16671 is efficacious in rats and appears safe.
副干酪乳杆菌 DSMZ16671，即使是热死的，也能敏感地与变形链球菌共聚。变形链球菌强烈参与人类和啮齿动物的龋齿诱导。我们假设：（1）如果在建立的龋齿模型中给大鼠喂食这些乳杆菌，大鼠牙齿中的变形链球菌恢复量会减少，相应的龋齿也会减少；（2）这些乳杆菌的毒理学检测将表明它们是良性的。形成了四组特定病原体无大鼠组：变形链球菌 10449S 接种/16671 补充饮食；未接种/16671 补充饮食；变形链球菌 10449S 接种/安慰剂饮食；未接种/安慰剂饮食。对热灭活 DSMZ16671 进行了毒性和致突变性的标准检测。在实验中期和末期，无论是接种变形链球菌的大鼠的变形链球菌回收量，还是大鼠的龋齿病变评分，与对照组相比，都显著降低。未检测到 DSMZ16671 的毒性或致突变性。使用热灭活的 DSMZ16671 在大鼠中是有效的，并且看起来是安全的。
