Institute of Medical Virology, Hospital of the Goethe University Frankfurt, Frankfurt am Main, Germany.
Sex Transm Dis. 2010 Jul;37(7):454-9. doi: 10.1097/OLQ.0b013e3181cfcc2b.
The objective of this study was to assess the seroprevalence of coinfecting viruses and Treponema pallidum (T. pallidum) in a cohort of 205 antiretrovirally treated HIV-infected individuals (152 females and 53 males, aged: 19-71 years) in rural Lesotho. Furthermore agent-specific immune responses were investigated by analyzing antibody titers against herpes simplex virus type 2 (HSV-2) and against T. pallidum.
Serum samples were tested by enzyme-linked immunosorbent assay for antibodies against HSV-2, cytomegalovirus, hepatitis A, B, and C viruses, and T. pallidum.
Seroprevalences (95% confidence intervals) were found to be 100% (98.5%-100%) for anti-cytomegalovirus, 98.5% (95.7%-99.7%) for anti-hepatitis A virus, 35.5% (28.9%-42.6%) for anti-HBc, 5.5% (2.8%-9.6%) for hepatitis B surface antigen, and 0.5% (0.0%-2.8%) for anti-hepatitis C virus. Only 78.5% (72.2%-84.0%) were anti-HSV-2 positive and 29.0% (22.8%-35.8%) had antibodies against T. pallidum. Only anti-HSV-2 titers showed gender- and CD4 cell-count dependent differences: females with >500 CD4 cells/microL had an average anti-HSV-2 titer of 446 compared with males of 398 AU/mL (not significant), but in those with 250 to 500 CD4 cells/microL, there was a significant difference with a mean titer of 467 compared to 302 AU/mL in males (P = 0.001).
A high seroprevalence of CMV, HAV, and HBV was found in both genders. One-third of the patients had been exposed to HBV and T. pallidum. The generally high HSV-2 prevalence showed gender- and CD4 cell count-dependent differences in HSV-2 antibody titer.
本研究旨在评估 205 名接受抗逆转录病毒治疗的艾滋病毒感染个体（152 名女性和 53 名男性，年龄 19-71 岁）中合并感染病毒和梅毒螺旋体（T. pallidum）的血清阳性率。在莱索托农村地区。此外，通过分析针对单纯疱疹病毒 2（HSV-2）和 T. pallidum 的抗体滴度来研究特定于试剂的免疫反应。
通过酶联免疫吸附试验检测血清样本中针对 HSV-2、巨细胞病毒、甲型肝炎、乙型肝炎和丙型肝炎病毒以及 T. pallidum 的抗体。
抗巨细胞病毒的血清阳性率（95%置信区间）为 100%（98.5%-100%），抗甲型肝炎病毒为 98.5%（95.7%-99.7%），抗乙型肝炎核心抗原为 35.5%（28.9%-42.6%），乙型肝炎表面抗原为 5.5%（2.8%-9.6%），丙型肝炎病毒为 0.5%（0.0%-2.8%）。仅 78.5%（72.2%-84.0%）为抗 HSV-2 阳性，29.0%（22.8%-35.8%）有抗梅毒螺旋体抗体。只有抗 HSV-2 滴度显示出性别和 CD4 细胞计数的依赖性差异：CD4 细胞/微升>500 的女性平均抗 HSV-2 滴度为 446，而男性为 398 AU/mL（无统计学意义），但在 CD4 细胞/微升 250 至 500 之间，存在明显差异，平均滴度为 467 与男性的 302 AU/mL 相比（P=0.001）。
在两性中均发现巨细胞病毒、甲型肝炎和乙型肝炎的高血清阳性率。三分之一的患者曾接触过乙型肝炎和梅毒螺旋体。普遍较高的 HSV-2 患病率在 HSV-2 抗体滴度方面显示出性别和 CD4 细胞计数的依赖性差异。
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