Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Mol Oral Microbiol. 2010 Jun;25(3):200-6. doi: 10.1111/j.2041-1014.2010.00572.x.
Aggregatibacter actinomycetemcomitans is usually isolated from the oral cavity where it is associated with active periodontitis. The species can be divided into six serotypes (a-f) according to their surface carbohydrate antigens. However, some clinical isolates cannot be grouped within these six serotypes. Gram-negative, facultative anaerobic, catalase-positive coccobacilli were isolated from a patient with periodontitis and identified by employing genetic, biochemical and serological analyses. Phenotypic data identified the isolate as A. actinomycetemcomitans. Serotype-specific polysaccharide antigen from the isolate was untypeable by immunodiffusion testing in comparison with reference A. actinomycetemcomitans serotype a to f strains. Biofilm formation by the isolate was strong but cytotoxic activity was low. Gas chromatography/mass spectroscopy analysis of partially methylated alditol acetates from surface polysaccharide showed the presence of 2,4-di-O-methyl-rhamnose and 2,3,6-tri-O-methyl-glucose, with a 1 : 1 m ratio. The (1)H- and (13)C-nuclear magnetic resonance spectra of the antigen showed that both constituent glycoses had alpha-anomeric configuration. It is proposed that the untyped strain is a new A. actinomycetemcomitans serotype, designated serotype g.
伴放线聚集杆菌通常从口腔中分离出来，与活动性牙周炎有关。根据其表面碳水化合物抗原，该物种可分为 6 个血清型（a-f）。然而，一些临床分离株不能归入这 6 个血清型。从一位牙周炎患者中分离出革兰氏阴性、兼性厌氧、触酶阳性的球杆菌，并通过遗传、生化和血清学分析进行鉴定。表型数据将分离株鉴定为伴放线聚集杆菌。与参考的伴放线聚集杆菌血清型 a 至 f 菌株相比，分离株的血清型特异性多糖抗原在免疫扩散试验中无法定型。该分离株的生物膜形成能力较强，但细胞毒性活性较低。表面多糖的部分甲基化醛糖醇乙酸酯的气相色谱/质谱分析显示存在 2,4-二-O-甲基-鼠李糖和 2,3,6-三-O-甲基-葡萄糖，比例为 1:1。抗原的 1H 和 13C 核磁共振谱表明，两种组成糖均具有α-端基构型。建议未定型菌株为新的伴放线聚集杆菌血清型，命名为血清型 g。
