印度尼西亚果蝠中新发现的副粘病毒的分子检测。
Molecular detection of a novel paramyxovirus in fruit bats from Indonesia.
机构信息
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
出版信息
Virol J. 2012 Oct 19;9:240. doi: 10.1186/1743-422X-9-240.
Abstract
BACKGROUND
Fruit bats are known to harbor zoonotic paramyxoviruses including Nipah, Hendra, and Menangle viruses. The aim of this study was to detect the presence of paramyxovirus RNA in fruit bats from Indonesia.
METHODS
RNA samples were obtained from the spleens of 110 fruit bats collected from four locations in Indonesia. All samples were screened by semi-nested broad spectrum reverse transcription PCR targeting the paramyxovirus polymerase (L) genes.
RESULTS
Semi-nested reverse transcription PCR detected five previously unidentified paramyxoviruses from six fruit bats. Phylogenetic analysis showed that these virus sequences were related to henipavirus or rubulavirus.
CONCLUSIONS
This study indicates the presence of novel paramyxoviruses among fruit bat populations in Indonesia.
摘要
背景
已知果蝠携带包括尼帕、亨德拉和曼纳干病毒在内的人畜共患副黏病毒。本研究旨在检测印度尼西亚果蝠中副黏病毒 RNA 的存在。
方法
从印度尼西亚四个地点采集的 110 只果蝠的脾脏中获得 RNA 样本。所有样本均通过针对副黏病毒聚合酶（L）基因的半巢式广谱逆转录 PCR 进行筛查。
结果
半巢式逆转录 PCR 从六只果蝠中检测到五种先前未鉴定的副黏病毒。系统发育分析表明，这些病毒序列与亨德拉病毒或 rubulavirus 有关。
结论
本研究表明印度尼西亚果蝠种群中存在新型副黏病毒。
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