Department of Oral and Maxillofacial Plastic Surgery, Martin-Luther-University Halle-Wittenberg, Halle, Germany.
Int J Biol Markers. 2010 Apr-Jun;25(2):87-92. doi: 10.1177/172460081002500205.
The critical molecular regulator of hypoxia is the hypoxia-inducible factor 1 alpha (HIF-1alpha). The prognostic impact of this regulator protein in oral squamous cell carcinomas (OSCC) has not been comprehensively investigated. The aim of this study was to analyze the expression of HIF-1alpha in 82 patients with OSCC and to correlate it with their disease-specific survival. Immunohistochemical staining for HIF-1alpha was performed on 82 OSCC specimens using a standard immunoperoxidase technique. The expression of HIF-1alpha was correlated with poor disease-specific survival for OSCC patients. Patients with negatively or weakly HIF-1alpha-expressing tumors had a survival rate of 80%, whereas the survival decreased to only 33.6% in case of moderate or strong expression. In multivariate Cox regression analysis, we found a 3.5-fold increased risk of tumor-related death when HIF-1alpha was strongly expressed (p=0.016) compared to negative or weak expression of HIF-1alpha. We suggest HIF-1alpha is an independent prognostic marker in OSCC. Immunohistochemical detection of HIF-1alpha appears to be useful in the diagnosis of OSCC and to provide prognostic information in addition to TNM stage and histological grade.
缺氧的关键分子调节因子是缺氧诱导因子 1 阿尔法（HIF-1alpha）。这种调节蛋白在口腔鳞状细胞癌（OSCC）中的预后影响尚未得到全面研究。本研究旨在分析 82 例 OSCC 患者中 HIF-1alpha 的表达，并将其与疾病特异性生存率相关联。使用标准免疫过氧化物酶技术对 82 例 OSCC 标本进行 HIF-1alpha 的免疫组织化学染色。HIF-1alpha 的表达与 OSCC 患者的不良疾病特异性生存率相关。阴性或弱阳性 HIF-1alpha 表达肿瘤患者的生存率为 80%，而中度或强表达时则降至 33.6%。在多变量 Cox 回归分析中，与 HIF-1alpha 阴性或弱阳性表达相比，当 HIF-1alpha 强表达时，肿瘤相关死亡的风险增加了 3.5 倍（p=0.016）。我们建议 HIF-1alpha 是 OSCC 的一个独立预后标志物。HIF-1alpha 的免疫组织化学检测似乎可用于 OSCC 的诊断，并提供除 TNM 分期和组织学分级以外的预后信息。
