Biomedical Engineering Department, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA.
Opt Lett. 2010 Jun 15;35(12):2094-6. doi: 10.1364/OL.35.002094.
We demonstrate the feasibility of estimating hemoglobin oxygen saturation (SaO(2)) from erythrocytes over a visible wavelength band using high-resolution spectroscopic optical coherence tomography (SOCT). We take SOCT spectra from individual cells on in vitro human blood phantoms and calculate SaO(2) by fitting the spectra to an analytical model. We also use diffusive spectroscopy (DS) measurements, which is validated by direct measurement of oxygen partial pressure, as a comparison. The result shows a reliable linear association between the SaO(2) derived from SOCT and the DS measurement, indicating this methodology has potential on in vivo applications to evaluate blood oxygenation.
我们使用高分辨率光谱光学相干断层扫描（SOCT）证明了在可见波长波段估算红细胞血红蛋白氧饱和度（SaO（2））的可行性。我们从体外人血模型中的单个细胞获取 SOCT 光谱，并通过将光谱拟合到分析模型来计算 SaO（2）。我们还使用扩散光谱（DS）测量值作为比较，该测量值通过直接测量氧分压进行验证。结果表明，SOCT 衍生的 SaO（2）与 DS 测量值之间存在可靠的线性关联，表明该方法具有在体内应用于评估血氧的潜力。
