Oh Byung Ho, Lee Yang Won, Choe Yong Beom, Ahn Kyu Joong
Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.
Ann Dermatol. 2010 May;22(2):149-55. doi: 10.5021/ad.2010.22.2.149. Epub 2010 May 17.
This case-control study concerns a molecular biological method based on the data gathered from a group of Korean subjects to examine the distribution of Malassezia yeasts in seborrheic dermatitis (SD) patients. Cultures for Malassezia yeasts were taken from the foreheads, cheeks and chests of 60 patients with SD and in 60 healthy controls of equivalent age.
The purpose of this study is to identify the relationship between certain species of Malassezia and SD. This was done by analyzing the differences in the distribution of Malassezia species in terms of age and body parts of the host with healthy controls.
26S rDNA PCR-RFLP, a fast and accurate molecular biological method, was used to overcome the limits of morphological and biochemical methods.
The positive Malassezia culture rate was 51.7% in patients with SD, which was lower than that of healthy adults (63.9%). M. restricta was dominant in patients with SD (19.5%). Likewise, M. restricta was identified as a common species (20.5%) in healthy controls. In the ages 31~40, M. restricta was found to be the most common species (31.6%) among SD patients.
According to the results of the study, the most frequently isolated species was M. restricta (19.5%) in patients with SD. There was no statistically significant difference in the distribution of Malassezia species between the SD patients and healthy control groups.
本病例对照研究涉及一种基于从一组韩国受试者收集的数据的分子生物学方法，用于检查马拉色菌在脂溢性皮炎（SD）患者中的分布情况。从60例SD患者以及60名年龄相当的健康对照者的额头、脸颊和胸部采集马拉色菌培养样本。
本研究的目的是确定某些马拉色菌物种与SD之间的关系。通过分析马拉色菌物种在宿主年龄和身体部位方面与健康对照者的分布差异来实现这一目的。
采用26S rDNA PCR-RFLP这一快速准确的分子生物学方法，以克服形态学和生化方法的局限性。
SD患者中马拉色菌培养阳性率为51.7%，低于健康成年人（63.9%）。局限马拉色菌在SD患者中占主导地位（19.5%）。同样，局限马拉色菌在健康对照者中也是常见物种（20.5%）。在31至40岁年龄段，局限马拉色菌是SD患者中最常见的物种（31.6%）。
根据研究结果，SD患者中最常分离出的物种是局限马拉色菌（19.5%）。SD患者与健康对照组之间马拉色菌物种的分布没有统计学上的显著差异。
