Tropical Pesticides Research Institute, Division of Livestock and Human Disease vector control, Mosquito Section, P,O, Box 3024, Arusha, Tanzania.
BMC Infect Dis. 2010 Jun 15;10:172. doi: 10.1186/1471-2334-10-172.
To ensure sustainable routine surveillance of mosquito vectors, simple, effective and ethically acceptable tools are required. As a part of that, we evaluated the efficiency of resting boxes baited with fresh and aging cattle urine for indoor and outdoor sampling of An. arabiensis in the lower Moshi rice irrigation schemes.
A cattle urine treatment and re-treatment schedule was used, including a box with a piece of cloth re-treated with urine daily, and once after 3 and 7 day. Resting box with piece of black cloth not treated with urine was used as a control. Each treatment was made in pair for indoor and outdoor sampling. A 4 by 4 Latin square design was used to achieve equal rotation of each of the four treatments across the experimental houses. Sampling was done over a period of 6 months, once per week.
A total of 7871 mosquitoes were collected throughout the study period. 49.8% of the mosquitoes were collected from resting box treated with urine daily; 21.6% and 20.0% were from boxes treated 3 and 7 days respectively. Only 8.6% were from untreated resting box (control). The proportion collected indoors was approximately 2 folds greater than the outdoor. Of all mosquitoes, 12.3% were unfed, 4.1% full fed, 34.2% semi-gravid and 49.4% gravid.
Fresh and decaying cattle urine odour baited resting boxes offer an alternative tool for sampling particularly semi-gravid and gravid An. arabiensis. Evaluation in low density seasons of An. arabiensis in different ecological settings remains necessary. This sampling method may be standardized for replacing human landing catch.
为确保对蚊虫媒介进行可持续的常规监测，需要使用简单、有效且符合伦理的工具。作为其中的一部分，我们评估了用新鲜和陈旧牛尿对下莫希水稻灌溉系统中室内和室外的致倦库蚊进行诱捕的效果。
我们采用了牛尿处理和再处理方案，包括一个每天用尿液处理一块布的盒子，以及在第 3 天和第 7 天各处理一次。一个未用尿液处理的黑色布块的诱蚊盒作为对照。室内和室外采样均采用成对的处理方法。采用 4×4 拉丁方设计，在实验房屋之间均匀分配每个处理的轮换。每周采样一次，持续 6 个月。
整个研究期间共收集了 7871 只蚊子。49.8%的蚊子来自每天用尿液处理的诱蚊盒；21.6%和 20.0%分别来自处理 3 天和 7 天的盒子。只有 8.6%的蚊子来自未处理的诱蚊盒（对照）。室内收集的蚊子比例大约是室外的两倍。在所有蚊子中，12.3%未进食，4.1%饱食，34.2%半饱血，49.4%饱血。
新鲜和腐败牛尿气味诱捕的诱蚊盒为采集致倦库蚊，特别是半饱血和饱血的致倦库蚊提供了一种替代工具。在不同生态环境中评估致倦库蚊低密度季节的情况仍然是必要的。这种采样方法可以标准化，以替代人工诱蚊捕集。
