Kweka Eliningaya J, Mwang'onde Beda J, Mahande Aneth M
Tropical Pesticides Research Institute, Division of Livestock and Human Diseases Vectors Control, Mosquito Section, P,O,Box 3024, Arusha, Tanzania.
Parasit Vectors. 2010 Aug 19;3:75. doi: 10.1186/1756-3305-3-75.
Odour baited resting boxes are simple, reliable and important tools for sampling malaria vector mosquitoes in surveillance and control programmes in different parts of Africa. To optimize the use of cow urine baited resting boxes for sampling An. arabiensis, a community-based study was conducted in Mabogini hamlet in the Lower Moshi irrigation scheme area.
Experimental designs using 3 by 3 Latin square were conducted for twenty days to evaluate the following: i) the effect of different parameters in the sampling of mosquitoes using odour baited resting boxes; ii) the performance of odour baited traps under indoor and outdoor conditions and the effect of people sleeping indoors on mosquito density; iii) the effect of position in the placement of traps on collection of mosquitoes; and, iv) the efficiency of the trap outdoors at three different distances from the house wall. One extra house served as the sentinel house to monitor species abundance using a CDC-miniature light trap.
8581 mosquitoes were sampled by odour baited resting boxes of which, 8051 (93.82%) were An. arabiensis and 530 (6.18%) Cx. quinquefasciatus. The light trap collected 12,420 mosquitoes, of which 9442 (76.02%) were An. arabiensis, 126 (1.01%) An. funestus group, 230 (1.85%) An. rufipes and 2622 (21.11%) Cx. quinquefasciatus. The best height for outdoor mosquitoes sampling was 15 cm and 220 cm while indoors was 105 cm. The difference in mosquito collection between different outdoor and indoor heights was statistically significant (p < 0.0001). The optimal outdoor location of odour baited resting boxes from the wall of the house was 3 m.
The results of these studies demonstrate an optimal method for sampling during surveillance and control programmes in rural villages of highlands and arid areas of Africa using inexpensive baits and boxes.
气味诱捕休息箱是非洲不同地区疟疾媒介蚊子监测和控制项目中简单、可靠且重要的采样工具。为优化使用牛尿诱捕休息箱对阿拉伯按蚊进行采样，在莫希下游灌溉区的马博吉尼小村庄开展了一项基于社区的研究。
采用3×3拉丁方实验设计，持续20天，以评估以下内容：i）使用气味诱捕休息箱采样蚊子时不同参数的影响；ii）气味诱捕器在室内和室外条件下的性能以及室内睡觉的人对蚊子密度的影响；iii）诱捕器放置位置对蚊子收集的影响；iv）诱捕器在离房屋墙壁三个不同距离处的户外效率。另外一所房屋用作哨兵房，使用疾控中心微型光诱捕器监测物种丰度。
通过气味诱捕休息箱共采集到8581只蚊子，其中8051只（93.82%）为阿拉伯按蚊，530只（6.18%）为致倦库蚊。光诱捕器收集到12420只蚊子，其中9442只（76.02%）为阿拉伯按蚊，126只（1.01%）为嗜人按蚊种群，230只（1.85%）为微红按蚊，2622只（21.11%）为致倦库蚊。户外蚊子采样的最佳高度为15厘米和220厘米，室内为105厘米。不同户外和室内高度的蚊子收集量差异具有统计学意义（p<0.0001）。气味诱捕休息箱离房屋墙壁的最佳户外位置为3米。
这些研究结果表明，在非洲高地和干旱地区的农村村庄进行监测和控制项目时，使用廉价诱饵和诱捕箱可得出一种最佳采样方法。
