Laboratoire de Biologie et Biotechnologie des Microorganismes, Pôle d'Excellence Régionale AUF, Faculté des Sciences Semlalia, Université Cadi Ayyad, BP 2390, 40000 Marrakech, Morocco.
Phytomedicine. 2010 Nov;17(13):1057-60. doi: 10.1016/j.phymed.2010.03.020.
The discovery of antifungal drugs had eradicated some infections that ravaged the humankind. But their indiscriminate use has led to the development of multidrug resistant pathogens. One strategy employed to overcome these resistance mechanisms is the use of combination of the essential oils (EOs) of medicinal plants and conventional drugs. In this study, we investigated a possible synergistic effect of the EOs of two Moroccan endemic thymes (Thymus broussonetii and T. maroccanus) with amphotericin B (Amp B) and fluconazol against Candida albicans. The fractional inhibitory concentration indices (FICI) of T. maroccanus and T. broussonetii EOs combined with Amp B and fluconazol, calculated from the checkerboard titer assay, were 0.49, 0.27, 0.37 and 0.3, respectively. Also, our results indicate that the synergistic effect of EOs with fluconazol was stronger than the combination with Amp B. All these data highlight that the EOs tested potentiate the antifungal action of Amp B and fluconazol, suggesting a possible utilization of these EOs in addition to antifungal drugs for the treatment of some candidiasis due to C. albicans. The use of these combinations is likely to reduce the minimum effective dose of the drugs, thus minimizing their toxic side effects and the treatment cost.
抗真菌药物的发现已经消除了一些肆虐人类的感染。但它们的滥用导致了多药耐药病原体的发展。克服这些耐药机制的一种策略是使用药用植物精油（EOs）与常规药物联合使用。在这项研究中，我们研究了两种摩洛哥特有百里香（Thymus broussonetii 和 T. maroccanus）的 EOs 与两性霉素 B（Amp B）和氟康唑联合使用对白色念珠菌的可能协同作用。棋盘微量滴定法计算的 T. maroccanus 和 T. broussonetii EOs 与 Amp B 和氟康唑联合使用的部分抑制浓度指数（FICI）分别为 0.49、0.27、0.37 和 0.3。此外，我们的结果表明，EOs 与氟康唑的协同作用强于与 Amp B 的组合。所有这些数据都表明，测试的 EOs 增强了 Amp B 和氟康唑的抗真菌作用，这表明这些 EOs 除了抗真菌药物外，可能还有用于治疗某些由白色念珠菌引起的念珠菌病的用途。这些组合的使用可能会降低药物的最小有效剂量，从而最大限度地减少其毒性副作用和治疗成本。
