肽和三烷基胺的水溶液导致意想不到的肽修饰。
Aqueous Solutions of Peptides and Trialkylamines Lead to Unexpected Peptide Modification.
机构信息
Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
出版信息
Molecules. 2021 Oct 27;26(21):6481. doi: 10.3390/molecules26216481.
Abstract
When trialkylamines are added to buffered solutions of peptides, unexpected adducts can be formed. These adducts correspond to Schiff base products. The source of the reaction is the unexpected presence of aldehydes in amines. The aldehydes can be detected in a few ways. Most importantly, they can lead to unanticipated results in proteomics experiments. Their undesirable effects can be minimized through the addition of other amines.
摘要
当三烷基胺被添加到缓冲的肽溶液中时，可能会形成意想不到的加合物。这些加合物对应于席夫碱产物。反应的来源是胺中意外存在的醛。醛可以通过几种方法检测到。最重要的是，它们会导致蛋白质组学实验中出现意想不到的结果。通过添加其他胺可以最小化它们的不良影响。
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