Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.
Virology. 2010 Sep 15;405(1):15-9. doi: 10.1016/j.virol.2010.05.024. Epub 2010 Jun 30.
Replication of avian influenza viruses (AIVs) in dogs may facilitate their adaptation in humans; however, the data to date on H5N1 influenza virus infection in dogs are conflicting. To elucidate the susceptibility of dogs to this pathogen, we infected two groups of 6 beagles with 10(6) 50% egg-infectious dose of H5N1 AIV A/bar-headed goose/Qinghai/3/05 (BHG/QH/3/05) intranasally (i.n.) and intratracheally (i.t.), respectively. The dogs showed disease symptoms, including anorexia, fever, conjunctivitis, labored breathing and cough, and one i.t. inoculated animal died on day 4 post-infection. Virus shedding was detected from all 6 animals inoculated i.n. and one inoculated i.t. Virus replication was detected in all animals that were euthanized on day 3 or day 5 post-infection and in the animal that died on day 4 post-infection. Our results demonstrate that dogs are highly susceptible to H5N1 AIV and may serve as an intermediate host to transfer this virus to humans.
禽流感病毒（AIVs）在狗体内的复制可能促进其在人类中的适应性；然而，目前关于 H5N1 流感病毒感染狗的数据存在矛盾。为了阐明狗对这种病原体的易感性，我们用 10（6）50%的鸡蛋感染剂量的 H5N1 AIV A/白头鹤/青海/3/05（BHG/QH/3/05）经鼻内（i.n.）和气管内（i.t.）分别感染两组 6 只比格犬。狗出现疾病症状，包括厌食、发热、结膜炎、呼吸困难和咳嗽，一只气管内接种的动物在感染后第 4 天死亡。从所有 6 只经鼻内接种的动物和 1 只经气管内接种的动物中均检测到病毒脱落。在感染后第 3 天或第 5 天安乐死的所有动物以及感染后第 4 天死亡的动物中均检测到病毒复制。我们的结果表明，狗对 H5N1 AIV 高度易感，可能成为将这种病毒传播给人类的中间宿主。
