Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, PR China.
Virus Res. 2013 Jul;175(1):52-7. doi: 10.1016/j.virusres.2013.04.004. Epub 2013 Apr 18.
Replication and transmission of avian influenza virus (AIV) in domestic dogs and cats may pose a risk to humans. The susceptibility of cats and dogs to H9N2 influenza virus was evaluated by intranasally or orally inoculating animals with an H9N2 influenza virus. Cats had recoverable virus in respiratory tissues and the olfactory bulb three days post-inoculation and shed H9N2 virus into nasal washes and pharyngeal swabs from day 2 through day 10 post-inoculation. Virus was recovered from respiratory tissues of dogs three days post-inoculation, but was not detected in nasal washes or pharyngeal swabs. While no virus shedding or replication was detected in cats or dogs following consumption of H9N2-infected chicks, one of two cats and one of two dogs seroconverted. Two of three naïve contact cats seroconverted following co-housing with cats that were intranasally inoculated with H9N2 virus, whereas none of the three naïve contact dogs seroconverted. Our results demonstrate that H9N2 AIV can infect domestic cats and dogs via the upper respiratory tract and indicate that cats are more susceptible than dogs to H9N2 AIV. These findings suggest that domestic dogs and cats may serve as host species contributing to the adaptation of H9N2 viruses in mammals.
禽流感病毒（AIV）在家犬和猫体内的复制和传播可能对人类构成威胁。通过鼻腔内或口服接种 H9N2 流感病毒来评估猫和狗对 H9N2 流感病毒的易感性。感染后第 3 天，猫的呼吸道组织和嗅球中可检测到可恢复的病毒，第 2 天至第 10 天可从鼻洗液和咽拭子中排出 H9N2 病毒。感染后第 3 天，狗的呼吸道组织中可检测到病毒，但鼻洗液或咽拭子中未检测到。虽然在食用感染 H9N2 的鸡雏后，猫或狗均未检测到病毒脱落或复制，但有 2 只猫和 2 只狗中的 1 只发生了血清学转换。与鼻腔内接种 H9N2 病毒的猫同居后，3 只未感染的接触猫中有 2 只发生了血清学转换，而 3 只未感染的接触狗中均未发生血清学转换。我们的结果表明，H9N2 AIV 可通过上呼吸道感染家猫和犬，并表明猫比狗更易感染 H9N2 AIV。这些发现表明，家犬和猫可能是宿主物种之一，有助于 H9N2 病毒在哺乳动物中的适应。
