Okwuzu Jane Ogoamaka, Odeiga Peter, AdetoroOtubanjo Olubunmi, Ezechi Oliver Chukwujekwu
Clinical Sciences Division, Nigerian Institute of Medical Research, Yaba Lagos.
Department of cell biology and genetics, University of Lagos, Akoka, Yaba Lagos.
Afr Health Sci. 2017 Mar;17(1):147-153. doi: 10.4314/ahs.v17i1.19.
The unrefined nature of the herbal preparations from (VA) and toxicity potentials of Sniper may both have severe consequences on the biochemical and genetic systems.
To assess the microscopic and macroscopic effects of these substances.
VA leafs and Sniper were prepared and dissolved in distilled water to give different concentrations. Series of baseline tests were carried out to establish concentration range for root growth. Series of twelve onion bulbs of three per series was prepared, with a series of three onion bulbs serving as control. Chromosomal aberrations were statistically analysed using chi- squared test. Root bundle mean length was obtained after 96 hours and EC values at 95% confidence interval was determined from a plot of root length against sample concentrations using Microsoft Excel software.
Total cytotoxic effect was induced by 2% sniper and 70% VA. EC for VA and sniper were 33.07 and 0.346 respectively. The two substances induced chromosomal aberrations and the effect was concentration dependent.
There are risks of these widely used substances for therapeutic and environmental purposes.
来自（VA）的草药制剂的未精制性质以及Sniper的毒性潜力可能对生化和遗传系统都产生严重后果。
评估这些物质的微观和宏观影响。
制备VA叶和Sniper，并将其溶解在蒸馏水中以得到不同浓度。进行了一系列基线测试以确定根生长的浓度范围。制备了一系列十二个洋葱鳞茎，每个系列三个，其中一系列三个洋葱鳞茎用作对照。使用卡方检验对染色体畸变进行统计学分析。96小时后获得根束平均长度，并使用Microsoft Excel软件从根长度对样品浓度的图中确定95%置信区间的EC值。
2%的Sniper和70%的VA诱导了总细胞毒性作用。VA和Sniper的EC分别为33.07和0.346。这两种物质诱导了染色体畸变，且作用呈浓度依赖性。
这些广泛用于治疗和环境目的的物质存在风险。
