Department of Pathology, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
Int J Colorectal Dis. 2010 Oct;25(10):1201-9. doi: 10.1007/s00384-010-1009-3. Epub 2010 Jul 13.
Astrocyte elevated gene-1 (AEG-1), as an HIV-1 or TNF-alpha-inducible transcript, is associated with various aspects of tumor malignancy. However, relatively little knowledge is available related to the role of AEG-1 in colorectal carcinoma.
By immunohistochemical and western blot analysis, we investigated AEG-1 expression in normal mucosa, adenomas, and carcinomas of colorectum. By statistical analysis, we determined its relationship with clinicopathological parameters and overall survival in colorectal carcinoma.
We found that AEG-1 expression levels were gradually elevated in normal tissues, low-grade adenoma, high-grade adenoma, and colorectal carcinoma, respectively. Though AEG-1 staining mainly emerged in the cytoplasm, we observed that nuclear staining of AEG-1 tends to become more common in lesions from patients with more advanced disease stages. Furthermore, there was a similar trend for Ki67 expression (as a proliferative index) from normal mucous to adenoma and carcinoma. Statistical analysis revealed that AEG-1 expression was markedly correlated with the UICC stage (P < 0.001), T classification (P = 0.002), N classification (P = 0.015), M classification (P = 0.010), Ki67 expression (P = 0.012), and histological differentiation (P = 0.037) in the colorectal cancer patients. Besides, those patients with high AEG-1 levels had shorter survival time (P < 0.001).
High AEG-1 expression correlates with poor overall survival in the colorectal carcinoma patients. In addition, AEG-1 expression in colorectal carcinoma may be associated with tumor progression, indicating that AEG-1 may be a potential preventive and chemotherapeutic target in the patients.
星形细胞上调基因-1（AEG-1）是 HIV-1 或 TNF-α诱导的转录物，与肿瘤恶性的各个方面有关。然而，关于 AEG-1 在结直肠癌中的作用，相关知识相对较少。
通过免疫组织化学和 Western blot 分析，我们研究了 AEG-1 在正常黏膜、腺瘤和结直肠癌中的表达。通过统计学分析，我们确定了 AEG-1 与结直肠癌临床病理参数和总生存期的关系。
我们发现 AEG-1 的表达水平在正常组织、低级别腺瘤、高级别腺瘤和结直肠癌中逐渐升高。尽管 AEG-1 染色主要出现在细胞质中，但我们观察到核染色的 AEG-1 在疾病进展阶段较高的患者中更为常见。此外，Ki67 表达（作为增殖指数）也呈现出从正常黏膜到腺瘤和癌的相似趋势。统计分析显示，AEG-1 的表达与 UICC 分期（P < 0.001）、T 分类（P = 0.002）、N 分类（P = 0.015）、M 分类（P = 0.010）、Ki67 表达（P = 0.012）和组织学分化（P = 0.037）显著相关。此外，AEG-1 水平较高的患者生存时间较短（P < 0.001）。
AEG-1 高表达与结直肠癌患者的总生存期不良相关。此外，AEG-1 在结直肠癌中的表达可能与肿瘤进展有关，这表明 AEG-1 可能是患者潜在的预防和化疗靶点。
