Department of Colorectal Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
Med Oncol. 2012 Dec;29(4):2858-63. doi: 10.1007/s12032-012-0186-z. Epub 2012 Feb 16.
The aim of the study was to investigate the role of AEG-1 in colorectal cancer and the relationship between AEG-1 protein expression and pathological characteristics and prognosis in colorectal cancer. The AEG-1 mRNA and protein expression level between the tumor cells from the cases with liver metastasis and those from the ones without live metastasis were detected by RT-PCR and immunohistochemistry staining. The relationship between AEG-1 and clinicopathological parameters of colorectal cancer was determined. AEG-1 expressed positively in 218 (41.92%) of the 520 cases examined. It showed that both mRNA and protein level AEG-1 were over-expressed in the cases with liver metastasis compared with those without liver metastasis (P=0.01). After immunohistochemistry analysis, the expression of AEG-1 protein was related to age, Duke's stage and distant metastasis (P=0.001, 0.001 and 0.016, respectively). After survival analysis, the cases with highly expressed AEG-1 protein attained a significantly poorer postoperative disease-specific survival than those with none/low-expressed AEG-1 protein (P=0.001). In the Cox regression test, histological grade, Duke's stage and AEG-1 were detected as the independent prognostic factors (P=0.035, 0.001 and 0.010, respectively). AEG-1 protein may be a potential new early liver metastasis biomarker of colorectal cancer.
本研究旨在探讨 AEG-1 在结直肠癌中的作用，以及 AEG-1 蛋白表达与结直肠癌病理特征和预后的关系。通过 RT-PCR 和免疫组化染色检测有肝转移和无肝转移病例肿瘤细胞中 AEG-1mRNA 和蛋白的表达水平，确定 AEG-1 与结直肠癌临床病理参数的关系。在 520 例检查病例中，有 218 例（41.92%）呈 AEG-1 阳性表达。结果表明，与无肝转移病例相比，有肝转移病例中 AEG-1 的 mRNA 和蛋白水平均过度表达（P=0.01）。经免疫组化分析，AEG-1 蛋白的表达与年龄、Duke 分期和远处转移有关（P=0.001、0.001 和 0.016）。通过生存分析，AEG-1 蛋白高表达的病例术后疾病特异性生存率明显低于 AEG-1 蛋白无/低表达的病例（P=0.001）。在 Cox 回归检验中，组织学分级、Duke 分期和 AEG-1 被检测为独立的预后因素（P=0.035、0.001 和 0.010）。AEG-1 蛋白可能是结直肠癌潜在的新的早期肝转移生物标志物。
