Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium.
J Appl Microbiol. 2010 Nov;109(5):1745-52. doi: 10.1111/j.1365-2672.2010.04805.x. Epub 2010 Jul 22.
The yeast Saccharomyces boulardii is used as a probiotic for the prevention and treatment of diarrhoea. In this study, the quality of 15 probiotic products containing S. boulardii was verified.
Using microsatellite typing, the identity of all Saccharomyces strains in the products was confirmed as S. boulardii. Additionally, solid-phase cytometry (SPC) and a plate method were used to enumerate S. boulardii cells. SPC was not only able to produce results more rapidly than plating (4h compared to 48h) but the cell counts obtained with SPC were significantly higher than the plate counts. Finally, we found that <1% of the S. boulardii cells survived 120min in gastric conditions and storage for 3months at 40°C with 75% relative humidity.
We developed a SPC method for the quantification of viable S. boulardii cells in probiotics. Additionally, we demonstrated that gastric conditions and storage have a marked effect on the viability of the yeast cells.
To our knowledge, this is the first time SPC is used for the quality control of probiotics with S. boulardii. Additionally, we demonstrated the need for gastric protection and accurate storage.
酿酒酵母布拉氏酵母被用作预防和治疗腹泻的益生菌。在本研究中，验证了含有布拉氏酵母的 15 种益生菌产品的质量。
使用微卫星分型，确认产品中所有酿酒酵母菌株的身份均为布拉氏酵母。此外，还使用固相细胞术（SPC）和平板法来计数布拉氏酵母细胞。SPC 不仅比平板法更快地产生结果（4 小时与 48 小时相比），而且 SPC 获得的细胞计数明显高于平板计数。最后，我们发现，在胃酸条件下，只有<1%的布拉氏酵母细胞能存活 120 分钟，在 40°C 和 75%相对湿度下储存 3 个月后，酵母细胞的存活率显著降低。
我们开发了一种用于定量益生菌中活布拉氏酵母细胞的 SPC 方法。此外，我们证明了胃酸条件和储存对酵母细胞活力有显著影响。
据我们所知，这是首次将 SPC 用于含有布拉氏酵母的益生菌的质量控制。此外，我们证明了需要进行胃保护和准确储存。
