• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Genome-wide real-time PCR for West Nile virus reduces the false-negative rate and facilitates new strain discovery.全基因组实时 PCR 检测西尼罗河病毒可降低假阴性率并有助于发现新的病毒株。
J Virol Methods. 2010 Oct;169(1):103-11. doi: 10.1016/j.jviromet.2010.07.005. Epub 2010 Jul 14.
2
Real time PCR assay for detection of all known lineages of West Nile virus.用于检测西尼罗河病毒所有已知谱系的实时聚合酶链反应检测法。
J Virol Methods. 2016 Oct;236:266-270. doi: 10.1016/j.jviromet.2016.07.026. Epub 2016 Jul 30.
3
A novel quantitative multiplex real-time RT-PCR for the simultaneous detection and differentiation of West Nile virus lineages 1 and 2, and of Usutu virus.一种新型的定量多重实时 RT-PCR 方法，可用于同时检测和区分西尼罗河病毒谱系 1 和 2 以及乌舒鲁病毒。
J Virol Methods. 2013 May;189(2):321-7. doi: 10.1016/j.jviromet.2013.02.019. Epub 2013 Mar 7.
4
Highly sensitive TaqMan RT-PCR assay for detection and quantification of both lineages of West Nile virus RNA.用于检测和定量西尼罗河病毒RNA两个谱系的高灵敏度TaqMan逆转录聚合酶链反应检测法。
J Clin Virol. 2006 Jul;36(3):177-82. doi: 10.1016/j.jcv.2006.02.008. Epub 2006 May 3.
5
Detection of West Nile virus by real-time PCR in crows in northern provinces of Iran.实时 PCR 法在伊朗北部省份对乌鸦中西尼罗河病毒的检测。
J Vector Borne Dis. 2020 Jan-Mar;57(1):37-39. doi: 10.4103/0972-9062.308797.
6
Rapid molecular detection and genotyping of West Nile Virus lineages 1 and 2 by real time PCR and melting curve analysis.通过实时PCR和熔解曲线分析对西尼罗河病毒1型和2型进行快速分子检测和基因分型。
J Virol Methods. 2014 Oct;207:54-9. doi: 10.1016/j.jviromet.2014.06.020. Epub 2014 Jun 30.
7
Application of a full-genome microarray-based assay for the study of genetic variability of West Nile virus.应用全基因组微阵列分析方法研究西尼罗河病毒的遗传变异。
J Virol Methods. 2012 Aug;183(2):219-23. doi: 10.1016/j.jviromet.2012.04.001. Epub 2012 Apr 25.
8
Diagnosis of West Nile virus infection in horses.马西尼罗河病毒感染的诊断
J Vet Diagn Invest. 2004 Jan;16(1):2-10. doi: 10.1177/104063870401600102.
9
Detection of West Nile virus lineages 1 and 2 by real-time PCR.通过实时聚合酶链反应检测西尼罗河病毒1型和2型谱系
J Virol Methods. 2007 Dec;146(1-2):355-8. doi: 10.1016/j.jviromet.2007.05.021. Epub 2007 Jun 29.
10
Simultaneous detection of West Nile and Japanese encephalitis virus RNA by duplex TaqMan RT-PCR.双重 TaqMan RT-PCR 法同时检测西尼罗河病毒和日本脑炎病毒 RNA。
J Virol Methods. 2013 Nov;193(2):554-7. doi: 10.1016/j.jviromet.2013.07.025. Epub 2013 Jul 25.

引用本文的文献

1
Evolutionary dynamics and molecular epidemiology of in New York State: 1999-2015.纽约州1999 - 2015年的进化动力学与分子流行病学
Virus Evol. 2019 Jul 21;5(2):vez020. doi: 10.1093/ve/vez020. eCollection 2019 Jul.
2
Parasite Tolerance and Host Competence in Avian Host Defense to West Nile Virus.鸟类宿主对西尼罗河病毒防御中的寄生虫耐受性与宿主能力
Ecohealth. 2018 Jun;15(2):360-371. doi: 10.1007/s10393-018-1332-7. Epub 2018 Mar 22.
3
Diagnosis of west nile virus human infections: overview and proposal of diagnostic protocols considering the results of external quality assessment studies.西尼罗河病毒人类感染的诊断：综合考虑外部质量评估研究结果的诊断方案概述及建议。
Viruses. 2013 Sep 25;5(10):2329-48. doi: 10.3390/v5102329.
4
Development of a multi-target TaqMan assay to detect eastern equine encephalitis virus variants in mosquitoes.用于检测蚊子中东马脑炎病毒变体的多靶点TaqMan检测法的开发。
Vector Borne Zoonotic Dis. 2012 Oct;12(10):872-6. doi: 10.1089/vbz.2012.1008. Epub 2012 Jul 26.
5
Recent progress in West Nile virus diagnosis and vaccination.西尼罗河病毒诊断与疫苗接种的最新进展。
Vet Res. 2012 Mar 1;43(1):16. doi: 10.1186/1297-9716-43-16.

本文引用的文献

1
Baboon model for West Nile virus infection and vaccine evaluation.用于西尼罗河病毒感染和疫苗评估的狒狒模型。
Virology. 2006 Nov 10;355(1):44-51. doi: 10.1016/j.virol.2006.06.033. Epub 2006 Aug 10.
2
Comparison of commercially available and novel West Nile virus immunoassays for detection of seroconversion in pig-tailed macaques (Macaca nemestrina).用于检测食蟹猴（Macaca nemestrina）血清转化的市售和新型西尼罗河病毒免疫测定法的比较。
Comp Med. 2006 Feb;56(1):46-54.
3
Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability.更快的定量实时PCR方案可能会降低灵敏度并显示出更高的变异性。
Nucleic Acids Res. 2005 Nov 27;33(21):e182. doi: 10.1093/nar/gni181.
4
Phylogenetic analysis of North American West Nile virus isolates, 2001-2004: evidence for the emergence of a dominant genotype.2001 - 2004年北美西尼罗河病毒分离株的系统发育分析：优势基因型出现的证据
Virology. 2005 Nov 25;342(2):252-65. doi: 10.1016/j.virol.2005.07.022. Epub 2005 Aug 31.
5
Methylene blue photoinactivation abolishes West Nile virus infectivity in vivo.亚甲蓝光灭活可消除西尼罗河病毒在体内的传染性。
Antiviral Res. 2005 Nov;68(2):84-7. doi: 10.1016/j.antiviral.2005.07.001. Epub 2005 Aug 9.
6
Epidemiology and transmission dynamics of West Nile virus disease.西尼罗河病毒病的流行病学与传播动力学
Emerg Infect Dis. 2005 Aug;11(8):1167-73. doi: 10.3201/eid1108.050289a.
7
Screening the blood supply for West Nile virus RNA by nucleic acid amplification testing.通过核酸扩增检测对血液供应进行西尼罗河病毒RNA筛查。
N Engl J Med. 2005 Aug 4;353(5):460-7. doi: 10.1056/NEJMoa044029.
8
West Nile virus among blood donors in the United States, 2003 and 2004.2003年和2004年美国献血者中的西尼罗河病毒
N Engl J Med. 2005 Aug 4;353(5):451-9. doi: 10.1056/NEJMoa044333.
9
Whole-genome transcription profiling of rhesus monkey rhadinovirus.恒河猴疱疹病毒的全基因组转录谱分析。
J Virol. 2005 Jul;79(13):8637-50. doi: 10.1128/JVI.79.13.8637-8650.2005.
10
West Nile virus infection after cardiac transplantation.心脏移植后感染西尼罗河病毒
J Heart Lung Transplant. 2005 Jun;24(6):774-6. doi: 10.1016/j.healun.2003.10.026.

全基因组实时 PCR 检测西尼罗河病毒可降低假阴性率并有助于发现新的病毒株。

Genome-wide real-time PCR for West Nile virus reduces the false-negative rate and facilitates new strain discovery.

机构信息

Department of Pathology, U. Oklahoma Health Sciences Center, 1100 N. Lindsay, Oklahoma City, OK 73104, United States.

出版信息

J Virol Methods. 2010 Oct;169(1):103-11. doi: 10.1016/j.jviromet.2010.07.005. Epub 2010 Jul 14.

DOI:10.1016/j.jviromet.2010.07.005
PMID:20637239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3189403/
Abstract

West Nile virus (WNV) causes significant morbidity and mortality worldwide. Transplant and transfusion recipients as well as the elderly are particularly at risk. WNV shows strain variation from season to season and from locale to locale. This poses a significant problem for diagnosis. Most assays use a single primer pair to detect WNV by QPCR, and can fail to detect novel stains. To overcome this limitation, a genome-wide, multiple primer-based real-time QPCR assay was developed for WNV. The same assay can be used for quantitation, viral variant discovery as well as for amplification of the entire viral genome using a single annealing temperature. It improves upon routine diagnosis as well as facilitates laboratory investigations of the pathology of WNV.

摘要

西尼罗河病毒（WNV）在全球范围内造成了重大的发病率和死亡率。移植和输血受者以及老年人尤其处于危险之中。WNV 显示出季节性和地域性的毒株变异。这给诊断带来了重大问题。大多数检测方法使用单个引物对通过 QPCR 检测 WNV，并且可能无法检测到新型菌株。为了克服这一限制，开发了一种基于全基因组、多引物的实时 QPCR 检测方法用于检测 WNV。该检测方法可用于定量、病毒变异发现以及使用单一退火温度扩增整个病毒基因组。它提高了常规诊断的水平，并为研究 WNV 的病理学提供了便利。