全基因组实时 PCR 检测西尼罗河病毒可降低假阴性率并有助于发现新的病毒株。
Genome-wide real-time PCR for West Nile virus reduces the false-negative rate and facilitates new strain discovery.
机构信息
Department of Pathology, U. Oklahoma Health Sciences Center, 1100 N. Lindsay, Oklahoma City, OK 73104, United States.
出版信息
J Virol Methods. 2010 Oct;169(1):103-11. doi: 10.1016/j.jviromet.2010.07.005. Epub 2010 Jul 14.
Abstract
West Nile virus (WNV) causes significant morbidity and mortality worldwide. Transplant and transfusion recipients as well as the elderly are particularly at risk. WNV shows strain variation from season to season and from locale to locale. This poses a significant problem for diagnosis. Most assays use a single primer pair to detect WNV by QPCR, and can fail to detect novel stains. To overcome this limitation, a genome-wide, multiple primer-based real-time QPCR assay was developed for WNV. The same assay can be used for quantitation, viral variant discovery as well as for amplification of the entire viral genome using a single annealing temperature. It improves upon routine diagnosis as well as facilitates laboratory investigations of the pathology of WNV.
摘要
西尼罗河病毒（WNV）在全球范围内造成了重大的发病率和死亡率。移植和输血受者以及老年人尤其处于危险之中。WNV 显示出季节性和地域性的毒株变异。这给诊断带来了重大问题。大多数检测方法使用单个引物对通过 QPCR 检测 WNV，并且可能无法检测到新型菌株。为了克服这一限制，开发了一种基于全基因组、多引物的实时 QPCR 检测方法用于检测 WNV。该检测方法可用于定量、病毒变异发现以及使用单一退火温度扩增整个病毒基因组。它提高了常规诊断的水平，并为研究 WNV 的病理学提供了便利。
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