Department of Anatomy, Faculty of Health Sciences, School of Medicine, University of Pretoria, BMW Building, P.O. Box 2034, Pretoria 0001, South Africa.
J Thromb Thrombolysis. 2010 Nov;30(4):502-6. doi: 10.1007/s11239-010-0502-4.
Contraceptives and hormone replacement have been extensively used since the late 1950s. However, adverse effects are common and include an increased risk of cardiovascular diseases, including thrombo-embolic diseases. Previous research has shown that ultrastructure of fibrin networks may provide great insight regarding the thrombotic potential of patients. The current study investigates the scanning electron microscopy (SEM) ultrastructure of fibrin networks of individuals using oral contraceptive therapy as well as individuals using hormone replacement. We compare micrographs of these two groups with micrographs of young, healthy individuals not using oral contraception. Platelet rich plasma and thrombin was used to prepare the fibrin clots. Here we show that during contraceptive and hormone replacement use, a netted fibrin layer forms. We suggest that oestradiol use causes fibrin network changes and these changes can be seen using SEM technology. These changes may provide further evidence regarding the increased occurrence of thrombotic events during contraceptive and hormone replacement therapy.
避孕药和激素替代疗法自 20 世纪 50 年代末以来得到了广泛应用。然而，它们也存在常见的不良反应，包括心血管疾病风险增加，包括血栓栓塞性疾病。先前的研究表明，纤维蛋白网络的超微结构可能为了解患者的血栓形成潜力提供重要线索。本研究通过扫描电子显微镜（SEM）研究了使用口服避孕药和激素替代疗法的个体的纤维蛋白网络的超微结构，并与未使用口服避孕药的年轻健康个体的微观照片进行了比较。富血小板血浆和凝血酶用于制备纤维蛋白凝块。在这里，我们表明在使用避孕药和激素替代疗法期间，会形成网状纤维蛋白层。我们认为，雌二醇的使用会导致纤维蛋白网络的变化，这些变化可以使用 SEM 技术观察到。这些变化可能为避孕药和激素替代疗法期间血栓形成事件增加提供进一步的证据。
