Department of Chemistry and Biochemistry and the Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Curr Opin Chem Biol. 2010 Oct;14(5):608-15. doi: 10.1016/j.cbpa.2010.06.182. Epub 2010 Jul 17.
Researchers have been using DNA for the rational design and construction of nanoscale objects for nearly 30 years. Recently, 'scaffolded DNA origami' has emerged as one of the most promising assembly techniques in DNA nanotechnology with a broad range of applications. In the past two years alone, DNA origami has been used to assemble water-soluble probe tiles for label-free RNA hybridization, to study single-molecule chemical reactions, to probe distance-dependent multivalent ligand-protein binding effects, and to organize a variety of relevant molecules including proteins, carbon nanotubes, and metal nanoparticles. This review will recount the origin, evolution, and current status of this extremely versatile assembly technique.
研究人员已经将 DNA 用于近 30 年的纳米级物体的合理设计和构建。最近，“支架 DNA 折纸术”作为 DNA 纳米技术中最有前途的组装技术之一，具有广泛的应用。仅在过去两年中，DNA 折纸术就被用于组装水溶性探针瓦片以进行无标记 RNA 杂交，研究单分子化学反应，探测距离依赖性多价配体-蛋白质结合效应，以及组织各种相关分子，包括蛋白质、碳纳米管和金属纳米粒子。本文回顾了这种极其通用的组装技术的起源、发展和现状。
