利用 TAL 效应物核酸酶靶向 DNA 双链断裂。
Targeting DNA double-strand breaks with TAL effector nucleases.
机构信息
Department of Plant Pathology, Iowa State University, Ames, Iowa 50011, USA.
出版信息
Genetics. 2010 Oct;186(2):757-61. doi: 10.1534/genetics.110.120717. Epub 2010 Jul 26.
Abstract
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.
摘要
在体内切割特定 DNA 序列的工程化核酸酶是靶向诱变的有价值的试剂。在这里，我们报告了一类新的序列特异性核酸酶，它们是通过将转录激活因子样效应物（TALEs）融合到 FokI 内切酶的催化结构域而产生的。天然和定制的 TALE-核酸酶融合物将 DNA 双链断裂引导至特定的靶向位点。
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