Periodontal Research Group, School of Dentistry, College of Medical and Dental Sciences, University of Birmingham, St. Chads Queensway, Birmingham, B4 6NN, United Kingdom.
J Proteome Res. 2010 Sep 3;9(9):4732-44. doi: 10.1021/pr100446f.
The 21-day experimental gingivitis model, an established noninvasive model of inflammation in response to increasing bacterial accumulation in humans, is designed to enable the study of both the induction and resolution of inflammation. Here, we have analyzed gingival crevicular fluid, an oral fluid comprising a serum transudate and tissue exudates, by LC-MS/MS using Fourier transform ion cyclotron resonance mass spectrometry and iTRAQ isobaric mass tags, to establish meta-proteomic profiles of inflammation-induced changes in proteins in healthy young volunteers. Across the course of experimentally induced gingivitis, we identified 16 bacterial and 186 human proteins. Although abundances of the bacterial proteins identified did not vary temporally, Fusobacterium outer membrane proteins were detected. Fusobacterium species have previously been associated with periodontal health or disease. The human proteins identified spanned a wide range of compartments (both extracellular and intracellular) and functions, including serum proteins, proteins displaying antibacterial properties, and proteins with functions associated with cellular transcription, DNA binding, the cytoskeleton, cell adhesion, and cilia. PolySNAP3 clustering software was used in a multilayered analytical approach. Clusters of proteins that associated with changes to the clinical parameters included neuronal and synapse associated proteins.
21 天实验性龈炎模型是一种针对人类细菌积累增加而引发炎症的非侵入性模型，旨在研究炎症的诱导和消退。本研究采用 LC-MS/MS 结合傅里叶变换离子回旋共振质谱和 iTRAQ 同重同位素标记技术，分析了牙龈沟液（一种包含血清渗出物和组织渗出物的口腔液体），以建立健康年轻志愿者中蛋白质炎症诱导变化的元蛋白质组图谱。在实验性龈炎的过程中，我们鉴定了 16 种细菌和 186 种人类蛋白质。尽管鉴定的细菌蛋白质的丰度没有随时间变化，但检测到了梭杆菌的外膜蛋白。梭杆菌种以前与牙周健康或疾病有关。鉴定的人类蛋白质涵盖了广泛的隔室（细胞外和细胞内）和功能，包括血清蛋白、具有抗菌特性的蛋白质以及与细胞转录、DNA 结合、细胞骨架、细胞黏附和纤毛相关的功能的蛋白质。使用 PolySNAP3 聚类软件进行了多层次分析方法。与临床参数变化相关的蛋白质簇包括神经元和突触相关蛋白。
