Department of Chemistry, Texas A&M University, College Station, Texas 77840, USA.
J Am Chem Soc. 2010 Aug 25;132(33):11608-12. doi: 10.1021/ja1034107.
Protein thiocarboxylates are involved in the biosynthesis of thiamin, molybdopterin, thioquinolobactin, and cysteine. Sequence analysis suggests that this post-translational modification is widely distributed in bacteria. Here we describe the development of lissamine rhodamine B sulfonyl azide as a sensitive click reagent for the detection of protein thiocarboxylates and describe the use of this reagent to detect PdtH, a putative protein thiocarboxylate involved in the biosynthesis of the pyridine dithiocarboxylic acid siderophore, in the Pseudomonas stutzeri proteome.
蛋白质硫代羧酸酯参与硫胺素、钼喋呤、硫代喹啉肟和半胱氨酸的生物合成。序列分析表明，这种翻译后修饰在细菌中广泛分布。在这里，我们描述了 Lissamine rhodamine B 磺酰叠氮化物的开发，作为一种灵敏的点击试剂，用于检测蛋白质硫代羧酸酯，并描述了该试剂在假单胞菌属蛋白质组中用于检测 PdtH 的用途，PdtH 是一种参与吡啶二硫代羧酸铁载体生物合成的假定蛋白质硫代羧酸酯。
