Jardin-Mathé Olivia, Bonnel David, Franck Julien, Wisztorski Maxence, Macagno Eduardo, Fournier Isabelle, Salzet Michel
University of Lille 1, CNRS FRE 2933, MALDI Imaging team, Laboratoire de Neuroimmunologie des Annélides, Villeneuve d'Ascq, France.
J Proteomics. 2008 Aug 21;71(3):332-45. doi: 10.1016/j.jprot.2008.07.004. Epub 2008 Jul 12.
MITICS is a new software developed for MALDI imaging. We tried to render this software compatible with all types of instruments. MITICS is divided in two parts: MITICS control for data acquisition and MITICS Image for data processing and images reconstruction. MITICS control is available for Applied BioSystems MALDI-TOF instruments and MITICS Image for both Applied BioSystems and Bruker Daltonics ones. MITICS Control provides an interface to the user for setting the acquisition parameters for the imaging sequence, namely set instruments acquisition parameters, create the raster of acquisition and control post-acquisition data processing, and provide this settings to the automatic acquisition software of the MALDI instrument. MITICS Image ensures image reconstruction, files are first converted to XML files before being loaded in a database. In MITICS image we have chosen to implement different data representations and calculations for image reconstruction. MITICS Image uses three different representations that have shown to ease extraction of information from the whole data set. It also offers image reconstruction base either on the maximum peak intensity or the peak area. Image reconstruction is possible for single ions but also by summing signals of different ions. MITICS was validated on biological cases.
MITICS是一款为基质辅助激光解吸电离成像（MALDI成像）开发的新软件。我们试图使该软件与所有类型的仪器兼容。MITICS分为两部分：用于数据采集的MITICS控制和用于数据处理及图像重建的MITICS图像。MITICS控制适用于应用生物系统公司的MALDI-TOF仪器，而MITICS图像则适用于应用生物系统公司和布鲁克道尔顿公司的仪器。MITICS控制为用户提供了一个界面，用于设置成像序列的采集参数，即设置仪器采集参数、创建采集光栅并控制采集后的数据处理，并将这些设置提供给MALDI仪器的自动采集软件。MITICS图像确保图像重建，文件在加载到数据库之前首先转换为XML文件。在MITICS图像中，我们选择为图像重建实现不同的数据表示和计算方法。MITICS图像使用三种不同的表示方法，这些方法已证明有助于从整个数据集中提取信息。它还提供基于最大峰强度或峰面积的图像重建。不仅可以对单个离子进行图像重建，还可以通过对不同离子的信号求和来进行图像重建。MITICS已在生物案例上得到验证。
