体细胞杂交体表达的抗病毒抗体库。
Repertoire of antiviral antibodies expressed by somatic cell hybrids.
作者信息
Gerhard W, Croce C M, Lopes D, Koprowski H
出版信息
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1510-4. doi: 10.1073/pnas.75.3.1510.
Abstract
Fusion between P3 x 63 Ag8 mouse myeloma cells and spleen cells from BALB/c mice immunized with influenza type A or B or parainfluenza type 1 virus generated reproducibly antiviral antibody-producing somatic cell hybrids (hybridomas). Eleven hybridomas derived from spleen cells of mice immunized with influenza type A virus were directed against the viral hemagglutinin, one reacted with a host component derived from chickens, and one expressed a specificity not further characterized. The hybridoma antibodies tended to be highly specific for the hemagglutinin of the immunizing virus and seemed to express the same repertoire of strain-specific antibody reactivities as splenic precursor B cells, they did not express any of the frequently occurring crossreactive anti-hemagglutinin specificities. Hybridomas producing crossreactive antibodies against hemagglutinin could be obtained if priming and boosting virus were heterologous.
摘要
将P3 x 63 Ag8小鼠骨髓瘤细胞与用甲型或乙型流感病毒或1型副流感病毒免疫的BALB/c小鼠的脾细胞进行融合，可重复性地产生抗病毒抗体的体细胞杂种（杂交瘤）。从用甲型流感病毒免疫的小鼠脾细胞中获得的11个杂交瘤针对病毒血凝素，1个与来源于鸡的宿主成分发生反应，1个表现出未进一步鉴定的特异性。杂交瘤抗体往往对免疫病毒的血凝素具有高度特异性，并且似乎表达与脾前体B细胞相同的菌株特异性抗体反应谱，它们不表达任何常见的交叉反应性抗血凝素特异性。如果初次免疫和加强免疫的病毒是异源的，则可以获得产生针对血凝素的交叉反应性抗体的杂交瘤。
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