Keydar I, Mesa-Tejada R, Ramanarayanan M, Ohno T, Fenoglio C, Hu R, Spiegelman S
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1524-8. doi: 10.1073/pnas.75.3.1524.
An indirect immunoperoxidase method is described, which can readily detect viral antigens in paraffin sections of primary, transplanted, and metastatic mammary tumors of mice. In addition to having the obvious advantage of not being limited to fresh specimens, immunoperoxidase staining of paraffin sections proved to be superior in many respects when compared with immunofluorescence and frozen sections. Immunoperoxidase staining of paraffin sections is permanent and provides the kind of histological detail required for precise cytological identification and localization with light microscopy. All of 25 tumors and 4 metastatic lesions showed evidence of glycoprotein gp52 as well as other mouse mammary tumor viral antigens. The pattern and intensity of the stain were related to the degree of histologic differentiation of the tumor. Wide variations in expression of viral antigens by individual malignant cells were observed within the same tumor.
本文描述了一种间接免疫过氧化物酶法，该方法可轻松检测小鼠原发性、移植性和转移性乳腺肿瘤石蜡切片中的病毒抗原。除了具有不限于新鲜标本这一明显优势外，与免疫荧光和冰冻切片相比，石蜡切片的免疫过氧化物酶染色在许多方面都更具优势。石蜡切片的免疫过氧化物酶染色是永久性的，可提供光学显微镜下精确细胞学鉴定和定位所需的组织学细节。25个肿瘤和4个转移灶均显示出糖蛋白gp52以及其他小鼠乳腺肿瘤病毒抗原的证据。染色的模式和强度与肿瘤的组织学分化程度相关。在同一肿瘤内，观察到单个恶性细胞的病毒抗原表达存在广泛差异。
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