College of Food and Bioengineering, South China University of Technology, Guangzhou, 510640, China.
Mini Rev Med Chem. 2010 Nov;10(13):1223-34. doi: 10.2174/13895575110091223.
Chromatography technology has been widely applied in various aspects of the pharmacy research on traditional Chinese medicine (TCM). This paper reviews literatures, published in the past decades, on the separation of active component from TCM using chromatography technology. Ultra-performance liquid chromatography (UPLC), high-speed counter-current chromatography (HSCCC), rapid resolution liquid chromatography (RRLC), supercritical fluid chromatography (SFC), affinity chromatography (AC), and bio-chromatography (BC) are introduced in detail. Compared to high performance of high-performance liquid chromatography (HPLC), analysis time and solvent loss are significantly reduced by UPLC with increase in resolution and sensitivity. Some ingredients from nature derived drugs can be separated more completely by HSCCC, which has remarkable characteristics such as low cost, simple operation and no pollution. Trace components from complex systems can be selectively and efficiently separated and purified by AC, This feature makes it effective in isolation and identification of active components of Chinese herbs. Interference of some impurities could be excluded by BC. Active ingredients that are difficult to be separated by normal method can be acquired by SFC. Currently, application of novel chromatography techniques in TCM is still in the exploratory stage and many problems, such as preparation of stationary phase and detection, need to be solved.
色谱技术已广泛应用于中药（TCM）药学研究的各个方面。本文综述了过去几十年中使用色谱技术从中药中分离活性成分的文献。详细介绍了超高效液相色谱（UPLC）、高速逆流色谱（HSCCC）、快速分辨液相色谱（RRLC）、超临界流体色谱（SFC）、亲和色谱（AC）和生物色谱（BC）。与高效液相色谱（HPLC）相比，UPLC 的分辨率和灵敏度提高，分析时间和溶剂损失显著减少。HSCCC 可更完全地分离天然药物中的一些成分，具有成本低、操作简单、无污染等显著特点。AC 可选择性、高效地分离和纯化复杂体系中的痕量成分，这一特点使其在中草药有效成分的分离和鉴定中非常有效。BC 可以排除一些杂质的干扰。一些难以用常规方法分离的有效成分可以通过 SFC 获得。目前，新型色谱技术在中药中的应用仍处于探索阶段，还需要解决固定相制备和检测等许多问题。
