Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada.
Environ Sci Technol. 2010 Aug 15;44(16):6068-73. doi: 10.1021/es100914r.
An extraction, separation, and purification method was developed for the identification and quantification of total bromine (TBr), extractable organobromine (EOBr), and five classes of identified EOBrs. Instrumental neutron activation analysis (INAA) was utilized to quantify EOBr and TBr. The method was then applied to liver samples of tuna, albatross, and polar bear collected from remote marine locations. Polybrominated biphenyls (PBBs), polybrominated diphenyl ethers (PBDEs), bromophenols (BRPs), hydroxylated (OH-) and methoxylated (MeO-) PBDEs were analyzed as identified EOBr. The majority of the bromine in these marine organisms was nonextractable or inorganic, with EOBr accounting for 10-28% of the TBr. Of the identified EOBr, in tuna and albatross, naturally occurring compounds, including MeO-PBDEs, OH-PBDEs, and BPRs, were prevalent. However, the identifiable EOBr in polar bears consisted primarily of synthetic compounds, including PBDEs and PBBs. Overall, 0.08-0.11% and 0.008-0.012% of EOBr and TBr, respectively, were identified. The proportion of EOBr that was identified in marine organisms was relatively small compared to the proportions for organofluorine and organochlorine compounds. This could be related to the great diversity of naturally occurring organobromine compounds in the environment. Naturally occurring brominated fatty acids were estimated to be the predominant compounds in the EOBr fraction.
开发了一种提取、分离和纯化方法，用于鉴定和定量总溴（TBr）、可提取有机溴（EOBr）和已鉴定的五类 EOBr。仪器中子活化分析（INAA）用于定量 EOBr 和 TBr。然后将该方法应用于从偏远海洋地区采集的金枪鱼、信天翁和北极熊的肝样。多溴联苯（PBBs）、多溴二苯醚（PBDEs）、溴酚（BRPs）、羟基（OH-）和甲氧基（MeO-）PBDEs 被分析为已鉴定的 EOBr。这些海洋生物中的大部分溴是非可提取的或无机的，EOBr 占 TBr 的 10-28%。在所鉴定的 EOBr 中，在金枪鱼和信天翁中，天然存在的化合物，包括 MeO-PBDEs、OH-PBDEs 和 BPRs，很常见。然而，在北极熊中可鉴定的 EOBr 主要由合成化合物组成，包括 PBDEs 和 PBBs。总的来说，分别有 0.08-0.11%和 0.008-0.012%的 EOBr 和 TBr 被鉴定。与有机氟和有机氯化合物相比，海洋生物中鉴定出的 EOBr 比例相对较小。这可能与环境中天然存在的有机溴化合物的多样性有关。天然存在的溴化脂肪酸估计是 EOBr 部分中的主要化合物。
