Tianjin Key Lab of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, P. R. China.
Analyst. 2010 Oct;135(10):2706-10. doi: 10.1039/c0an00237b. Epub 2010 Aug 11.
A novel aptamer biosensor for fast tetracyclines determination was established and illustrated in this paper. The tetracyclines aptamer as a specific affinity molecule was immobilized on the surface of the glassy carbon (GC) electrodes. It can specifically bind tetracyclines quickly in milk and other samples without sample treatment. Subsequently, the electrochemical signals are produced and measured accurately. The linear relationship between the currents and the tetracyclines concentration is 0.1-100 ng ml(-1). The sensitivity limit of the device is 1 ng ml(-1). The detection time is 5 min.
本文建立并阐述了一种用于快速测定四环素的新型适体生物传感器。该传感器将四环素适体作为一种特异性亲和分子固定在玻碳电极表面，无需样品处理即可快速特异性结合牛奶和其他样品中的四环素。随后，精确地产生和测量电化学信号。电流与四环素浓度之间存在 0.1-100ng/ml-1 的线性关系，该装置的检测限为 1ng/ml-1，检测时间为 5 分钟。
