Department of Biology, Technische Universität Darmstadt, 64287 Darmstadt, Germany.
Institute for Printing Science and Technology, Technische Universität Darmstadt, 64289 Darmstadt, Germany.
Biosensors (Basel). 2019 Jan 2;9(1):7. doi: 10.3390/bios9010007.
The excessive use of antibiotics in food-producing animals causes a steady rise of multiple antibiotic resistance in foodborne bacteria. Next to sulfonamides, the most common antibiotics groups are fluoroquinolones, aminoglycosides, and ß-lactams. Therefore, there is a need for a quick, efficient, and low-cost detection procedure for antibiotics. In this study, we propose an inkjet-printed aptamer-based biosensor developed for the detection of the fluoroquinolone ciprofloxacin. Due to their extraordinary high affinity and specificity, aptamers are already widely used in various applications. Here we present a ciprofloxacin-binding RNA aptamer developed by systematic evolution of ligands by exponential enrichment (SELEX). We characterized the secondary structure of the aptamer and determined the KD to 36 nM that allow detection of antibiotic contamination in a relevant range. We demonstrate that RNA aptamers can be inkjet-printed, dried, and resolved while keeping their functionality consistently intact. With this proof of concept, we are paving the way for a potential range of additional aptamer-based, printable biosensors.
在食用动物中过度使用抗生素会导致食源性病原体中多种抗生素耐药性的持续上升。除了磺胺类药物，最常见的抗生素类别是氟喹诺酮类、氨基糖苷类和β-内酰胺类。因此，需要一种快速、高效且低成本的抗生素检测方法。在本研究中，我们提出了一种基于喷墨打印的适体生物传感器，用于检测氟喹诺酮类环丙沙星。由于适体具有非凡的高亲和力和特异性，因此已广泛应用于各种应用中。在这里，我们展示了一种通过指数富集的配体系统进化（SELEX）开发的与环丙沙星结合的 RNA 适体。我们对适体的二级结构进行了表征，并确定了 KD 值为 36 nM，可在相关范围内检测抗生素污染。我们证明 RNA 适体可以进行喷墨打印、干燥和解析，同时保持其功能始终完好无损。通过这个概念验证，我们为潜在的一系列基于适体的可打印生物传感器铺平了道路。
