Department of Physiology, Faculty of Natural Sciences, Szczecin University, Al. Piastów 40b, 71-065, Szczecin, Poland.
Eur J Appl Physiol. 2010 Dec;110(6):1155-62. doi: 10.1007/s00421-010-1611-7. Epub 2010 Aug 17.
Maximal physical exertion is accompanied by increased degradation of purine nucleotides in muscles with the products of purine catabolism accumulating in the plasma. Thanks to membrane transporters, these products remain in an equilibrium between the plasma and red blood cells where they may serve as substrates in salvage reactions, contributing to an increase in the concentrations of purine nucleotides. In this study, we measured the concentrations of adenine nucleotides (ATP, ADP, AMP), inosine nucleotides (IMP), guanine nucleotides (GTP, GDP, GMP), and also pyridine nucleotides (NAD, NADP) in red blood cells immediately after standardized physical effort with increasing intensity, and at the 30th min of rest. We also examined the effect of muscular exercise on adenylate (guanylate) energy charge--AEC (GEC), and on the concentration of nucleosides (guanosine, inosine, adenosine) and hypoxanthine. We have shown in this study that a standardized physical exercise with increasing intensity leads to an increase in IMP concentration in red blood cells immediately after the exercise, which with a significant increase in Hyp concentration in the blood suggests that Hyp was included in the IMP pool. Restitution is accompanied by an increase in the ATP/ADP and ADP/AMP ratios, which indicates an increase in the phosphorylation of AMP and ADP to ATP. Physical effort applied in this study did not lead to changes in the concentrations of guanine and pyridine nucleotides in red blood cells.
最大的体力消耗伴随着嘌呤核苷酸在肌肉中的降解增加，嘌呤分解代谢的产物在血浆中积累。由于膜转运蛋白的存在，这些产物在血浆和红细胞之间保持着平衡，它们可以作为补救反应的底物，有助于增加嘌呤核苷酸的浓度。在这项研究中，我们测量了红细胞中腺嘌呤核苷酸（ATP、ADP、AMP）、肌苷核苷酸（IMP）、鸟嘌呤核苷酸（GTP、GDP、GMP）和吡啶核苷酸（NAD、NADP）的浓度，这些核苷酸在标准化的、强度递增的体力活动后立即测定，以及在休息 30 分钟时测定。我们还研究了肌肉运动对腺嘌呤（鸟嘌呤）能荷（AEC、GEC）以及核苷（鸟苷、肌苷、腺苷）和次黄嘌呤浓度的影响。本研究表明，随着强度的增加，标准化的体力活动会导致红细胞中 IMP 浓度立即增加，而血液中 Hyp 浓度的显著增加表明 Hyp 被纳入了 IMP 池。恢复过程伴随着 ATP/ADP 和 ADP/AMP 比值的增加，这表明 AMP 和 ADP 向 ATP 的磷酸化增加。本研究中施加的体力活动并没有导致红细胞中鸟嘌呤和吡啶核苷酸浓度的变化。
