Center for Molecular and Behavioral Neuroscience, Rutgers, The State University of New Jersey, Newark, New Jersey 07102, USA.
J Neurosci. 2010 Aug 18;30(33):11128-42. doi: 10.1523/JNEUROSCI.1327-10.2010.
A thorough knowledge of the intrinsic circuit properties of the entorhinal cortex (EC) and the temporal dynamics these circuits support is essential for understanding how information is exchanged between the hippocampus and neocortex. Using intracellular and extracellular recordings in the anesthetized rat and anatomical reconstruction of single cells, we found that EC5 and EC2 principal neurons form large axonal networks mainly within their layers, interconnected by the more vertically organized axon trees of EC3 pyramidal cells. Principal cells showed layer-specific unique membrane properties and contributed differentially to theta and gamma oscillations. EC2 principal cells were most strongly phase modulated by EC theta. The multiple gamma oscillators, present in the various EC layers, were temporally coordinated by the phase of theta waves. Putative interneurons in all EC layers fired relatively synchronously within the theta cycle, coinciding with the maximum power of gamma oscillation. The special wiring architecture and unique membrane properties of EC neurons may underlie their behaviorally distinct firing patterns in the waking animal.
深入了解内嗅皮层 (EC) 的内在电路特性以及这些电路支持的时间动态对于理解海马体和新皮层之间的信息交换至关重要。我们使用麻醉大鼠的细胞内和细胞外记录以及单个细胞的解剖重建，发现 EC5 和 EC2 主神经元主要在其层内形成大的轴突网络，由 EC3 锥体神经元更垂直组织的轴突树相互连接。主细胞表现出具有层特异性的独特膜特性，并对 theta 和 gamma 振荡做出不同的贡献。EC2 主细胞受 EC theta 的相位调制最强。存在于各种 EC 层中的多个 gamma 振荡器由 theta 波的相位进行时间协调。所有 EC 层中的假定中间神经元在 theta 周期内相对同步发射，与 gamma 振荡的最大功率一致。EC 神经元的特殊布线结构和独特的膜特性可能是其在清醒动物中行为表现不同的放电模式的基础。
