Center for Integrated Biotechnology and Department of Horticulture, Washington State University, Pullman, WA, USA.
Plant Signal Behav. 2010 Aug;5(8):991-4. doi: 10.4161/psb.5.8.12225. Epub 2010 Aug 1.
Recently we reported that CRLK1, a novel calcium/calmodulin-regulated receptor-like kinase plays an important role in regulating plant cold tolerance. Calcium/calmodulin binds to CRLK1 and upregulates its activity. Gene knockout and complementation studies revealed that CRLK1 is a positive regulator of plant response to chilling and freezing temperatures. Here we show that MEKK1, a member of MAP kinase kinase kinase family, interacts with CRLK1 both in vitro and in planta. The cold triggered MAP kinase activation in wild-type plants was abolished in crlk1 knockout mutants. Similarly, the cold induced expression levels of genes involved in MAP kinase signaling are also altered in crlk1 mutants. These results suggest that calcium/calmodulin-regulated CRLK1 modulates cold acclimation through MAP kinase cascade in plants.
最近我们报道称，CRLK1 是一种新型的钙/钙调素调节的受体样激酶，在调节植物耐寒性方面发挥着重要作用。钙/钙调素与 CRLK1 结合并上调其活性。基因敲除和互补研究表明，CRLK1 是植物对寒冷和冷冻温度响应的正调节剂。在这里，我们表明 MEKK1，一种 MAP 激酶激酶激酶家族的成员，在体外和体内都与 CRLK1 相互作用。在 crlk1 敲除突变体中，冷触发的 MAP 激酶激活在野生型植物中被消除。同样，在 crlk1 突变体中，也改变了与 MAP 激酶信号转导相关的基因的冷诱导表达水平。这些结果表明，钙/钙调素调节的 CRLK1 通过植物中的 MAP 激酶级联来调节冷驯化。
