Department of Chemistry, The Pennsylvania State University, 414 Wartik Laboratory, University Park, PA 16802, USA.
Curr Opin Chem Biol. 2010 Oct;14(5):644-51. doi: 10.1016/j.cbpa.2010.07.020. Epub 2010 Aug 20.
Recently, the hypothesis that protein motions are involved in enzymatic turnover has gained significant attention. We review cases where there is evidence that protein motions are rate-limiting in the overall catalytic cycle and examine experimental and theoretical evidence for how such motions enhance the probability of sampling the transition state configurations relative to the ground state. The impact of tunneling, the possible role of vibrational coupling and the value of conformational chemical landscapes are also scrutinized.
最近，蛋白质运动参与酶周转的假说引起了广泛关注。我们回顾了一些有证据表明蛋白质运动在整个催化循环中起限速作用的案例，并研究了实验和理论证据，以了解这些运动如何提高相对于基态采样过渡态构象的概率。我们还仔细研究了隧穿的影响、振动耦合的可能作用以及构象化学景观的价值。
