Sagami Chemical Research Institute, Ayase, Kanagawa 252-1193, Japan.
College of Agriculture Department of Life Science, Tamagawa University, Machida, Tokyo 194-8610, Japan.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):60-65. doi: 10.1099/ijs.0.038364-0. Epub 2012 Feb 10.
Two novel bacterial strains, designated Kc1(T) and Kc5(T), were isolated from soil in Japan. Cells of the novel strains were Gram-reaction-positive, aerobic or facultatively anaerobic, motile rods. Phylogenetic analyses based on 16S rRNA gene sequences indicated that both strains belonged to the genus Cellulomonas. The 16S rRNA gene sequences of strains Kc1(T) and Kc5(T) showed closest similarity to that of Cellulomonas terrae DB5(T) (98.1 % and 98.4 % similarity, respectively), and the 16S rRNA gene similarity between the two novel strains was 97.8 %. In both strains, the major menaquinone was MK-9(H(4)), the predominant polar lipids were diphosphatidylglycerol and phosphatidylinositol mannosides, and the peptidoglycan contained ornithine and glutamic acid. Cell-wall sugars were identified as rhamnose, galactose and mannose in strain Kc1(T) and rhamnose and glucose in strain Kc5(T). The DNA G+C contents of strains Kc1(T) and Kc5(T) were 73.6 mol% and 75.8 mol%, respectively. Based on the chemotaxonomic and physiological data and the results of DNA-DNA hybridizations, the two strains represent two novel species within the genus Cellulomonas, for which the names Cellulomonas soli sp. nov. (type strain Kc1(T) =DSM 24484(T) =JCM 17535(T)) and Cellulomonas oligotrophica sp. nov. (type strain Kc5(T) =DSM 24482(T) =JCM 17534(T)) are proposed.
两株新型细菌菌株，分别命名为 Kc1(T) 和 Kc5(T)，从日本土壤中分离得到。新菌株的细胞革兰氏反应阳性，为需氧或兼性厌氧、能动杆状。基于 16S rRNA 基因序列的系统发育分析表明，这两株菌均属于纤维单胞菌属。菌株 Kc1(T) 和 Kc5(T)的 16S rRNA 基因序列与纤维单胞菌属的地衣形纤维单胞菌（Cellulomonas terrae）DB5(T)最为相似（相似度分别为 98.1%和 98.4%），且两株新菌株之间的 16S rRNA 基因相似度为 97.8%。在这两株菌中，主要的menaquinone 是 MK-9(H(4))，主要的极性脂是双磷脂酰甘油和磷脂酰肌醇甘露糖苷，肽聚糖含有鸟氨酸和谷氨酸。细胞壁糖鉴定为 Kc1(T) 菌株中的鼠李糖、半乳糖和甘露糖和 Kc5(T) 菌株中的鼠李糖和葡萄糖。菌株 Kc1(T) 和 Kc5(T) 的 DNA G+C 含量分别为 73.6 mol%和 75.8 mol%。基于化学分类学和生理学数据以及 DNA-DNA 杂交的结果，这两株菌代表纤维单胞菌属中的两个新种，分别命名为土壤纤维单胞菌（Cellulomonas soli）sp. nov.（模式菌株 Kc1(T) =DSM 24484(T) =JCM 17535(T)）和寡营养纤维单胞菌（Cellulomonas oligotrophica）sp. nov.（模式菌株 Kc5(T) =DSM 24482(T) =JCM 17534(T)）。
