Mayfield C, Warnick G R, Albers J J
Clin Chem. 1979 Jul;25(7):1309-13.
Commercial heparin preparations (18 lots) from seven manufacturers were compared in the heparin-Mn2+ procedure for high-density-lipoprotein cholesterol quantitation. With normotriglyceridemic samples, 16 heparin lots, isolated from porcine intestinal mucosa, gave mean values for supernatant cholesterol that did not differ statistically; all were within 7 mg/L. Two heparin preparations from bovine lung gave results that were slightly (16 mg/L, average) but significantly (p less than 0.005) lower. With hypertriglyceridemic samples, we observed greater variation in supernatant cholesterol among the heparin preparations, which was ascribable to variable sedimentation by centrifugation of very-low-density and low-density lipoproteins precipitated by heparin-Mn2+ treatment. If the precipitated lipoproteins were completely removed by an ultrafiltration procedure, we saw no significant difference among the heparin preparations for results with hypertriglyceridemic samples.
对来自7家制造商的商用肝素制剂（18批），采用肝素-Mn2+法进行高密度脂蛋白胆固醇定量分析，并作比较。对于甘油三酯正常的样本，从猪肠黏膜分离得到的16批肝素，其上清液胆固醇的平均值无统计学差异；均在7mg/L以内。来自牛肺的两种肝素制剂的结果略低（平均为16mg/L），但差异显著（p<0.005）。对于甘油三酯过高的样本，我们观察到肝素制剂之间上清液胆固醇的差异更大，这归因于肝素-Mn2+处理沉淀的极低密度脂蛋白和低密度脂蛋白经离心后沉降情况不一。如果通过超滤程序完全去除沉淀的脂蛋白，那么对于甘油三酯过高的样本，各肝素制剂的结果无显著差异。
