Prionics, Wagistrasse 27a, 8952 Schlieren-Zurich, Switzerland.
Vet Rec. 2010 Aug 28;167(9):322-6. doi: 10.1136/vr.c3403.
In this study, interferon-gamma (IFN-gamma) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-gamma responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-gamma assay. Additionally, a tool named'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.
在这项研究中，比较了来自不同来源的结核菌素刺激全血培养中的干扰素-γ（IFN-γ）反应，以评估其在实验和自然感染牛分枝杆菌的牛中的诊断可靠性。对不同浓度的牛分枝杆菌和鸟分枝杆菌的纯化蛋白衍生物（PPD）进行了 IFN-γ反应的定量分析。刺激用的 PPD 来源和浓度之间存在显著差异（P<0.05），表明需要对 IFN-γ检测中使用的 PPD 进行标准化。此外，还定义了一个名为“相对效力 30”的工具，该工具可用于快速比较 PPD 的批次和来源。
