CSIRO Materials Science and Engineering, CSIRO Future Manufacturing Flagship, Clayton VIC 3168, Australia.
Langmuir. 2010 Oct 5;26(19):15399-408. doi: 10.1021/la101033n.
The relationship between the toxicity of zinc oxide (ZnO) nanoparticles (NPs) and their surface chemistry was investigated. Cytotoxicity, genotoxicity, and the ability to generate reactive oxygen species (ROS) were assessed for well-characterized ZnO NPs whose surface chemistry was varied from its pristine state by coating with oleic acid (OA), poly(methacrylic acid) (PMAA), or components adsorbed from cell culture medium (medium-soaked). It was found that uncoated NPs showed ROS accumulation and diminished cell viability whereas all tested surface coatings assisted in reducing ROS production and cytotoxicity. The ability of coatings to reduce the cytotoxicity of ZnO NPs was ranked in the following order: medium-soaked ≈ PMAA > OA. However, PMAA-coated ZnO had significant genotoxicity compared to uncoated ZnO and the other coated NPs, highlighting the need to investigate thoroughly the effects of NP surface modification on both cytotoxicity and genotoxicity assays. The lowest toxicity was achieved with a surface coating of components from a cell culture medium.
研究了氧化锌（ZnO）纳米粒子（NPs）的毒性与其表面化学性质之间的关系。用油酸（OA）、聚（甲基丙烯酸）（PMAA）或细胞培养基中吸附的成分（介质浸泡）对具有良好特性的 ZnO NPs 进行表面化学修饰，评估其细胞毒性、遗传毒性和生成活性氧物种（ROS）的能力。结果发现，未涂层的 NPs 表现出 ROS 积累和细胞活力降低，而所有测试的表面涂层都有助于减少 ROS 的产生和细胞毒性。涂层降低 ZnO NPs 细胞毒性的能力按以下顺序排列：介质浸泡≈PMAA＞OA。然而，与未涂层的 ZnO 和其他涂层 NPs 相比，PMAA 涂层的 ZnO 具有显著的遗传毒性，这突出表明需要深入研究 NP 表面改性对细胞毒性和遗传毒性测定的影响。采用细胞培养基成分的表面涂层可达到最低毒性。
