Division of Dermatology, Kobe University Graduate School of Medicine, Kobe, Japan.
J Dermatol Sci. 2010 Oct;60(1):21-8. doi: 10.1016/j.jdermsci.2010.08.002. Epub 2010 Aug 18.
Chemical peeling is one of the dermatological treatments available for certain cutaneous diseases and conditions or improvement of cosmetic appearance of photoaged skin.
We assessed the photochemopreventive effect of several clinically used chemical peeling agents on the ultraviolet (UV)-irradiated skin of hairless mice.
Chemical peeling was done using 35% glycolic acid dissolved in distilled water, 30% salicylic acid in ethanol, 10% or 35% trichloroacetic acid (TCA) in distilled water at the right back of UV-irradiated hairless mice every 2 weeks in case of glycolic acid, salicylic acid, and 10% TCA and every 4 weeks in case of 35% TCA for totally 18 weeks after the establishment of photoaged mice by irradiation with UVA+B range light three times a week for 10 weeks at a total dose of 420 J/cm(2) at UVA and 9.6 J/cm(2) at UVB. Tumor formation was assessed every week. Skin specimens were taken from treated and non-treated area for evaluation under microscopy, evaluation of P53 expression, and mRNA expression of cyclooxygenase (COX)-2. Serum level of prostaglandin E(2) was also evaluated.
All types of chemical peeling reduced tumor formation in treated mice, mostly in the treated area but also non-treated area. Peeling suppressed clonal retention of p53 positive abnormal cells and reduced mRNA expression of COX-2 in treated skin. Further, serum prostaglandin E(2) level was decreased in chemical peeling treated mice.
These results indicate that chemical peeling with glycolic acid, salicylic acid, and TCA could serve tumor prevention by removing photodamaged cells.
化学焕肤是皮肤科治疗某些皮肤疾病和状况或改善光老化皮肤外观的方法之一。
我们评估了几种临床使用的化学焕肤剂对无毛小鼠紫外线（UV）照射皮肤的光化学预防作用。
在每周照射 UVA+B 范围光 3 次、总剂量为 420 J/cm²的 UVA 和 9.6 J/cm²的 UVB 10 周后，建立光老化小鼠模型，然后每隔 2 周（对于甘醇酸、水杨酸和 10% TCA）或每隔 4 周（对于 35% TCA）使用 35%甘醇酸溶于蒸馏水中、30%水杨酸溶于乙醇中、10%或 35%三氯乙酸（TCA）溶于蒸馏水中对 UV 照射的无毛小鼠的右背部进行化学焕肤。总共进行 18 周。每周评估肿瘤形成情况。从处理和未处理区域取皮肤标本，在显微镜下进行评估、检测 P53 表达和环氧化酶（COX）-2 的 mRNA 表达，还评估了血清前列腺素 E（2）水平。
所有类型的化学焕肤均减少了处理小鼠的肿瘤形成，主要是在处理区域，但也在未处理区域。焕肤抑制了 p53 阳性异常细胞的克隆保留，并减少了处理皮肤中 COX-2 的 mRNA 表达。此外，化学焕肤处理的小鼠血清前列腺素 E（2）水平降低。
这些结果表明，甘醇酸、水杨酸和 TCA 的化学焕肤可以通过去除光损伤细胞来预防肿瘤。
