Calvo Tamara R, Demarco Diego, Santos Fabio V, Moraes Helen P, Bauab Taís M, Varanda Eliana A, Cólus Ilce M S, Vilegas Wagner
UNESP - São Paulo State University, Instituto de Química de Araraquara, Departamento de Química Orgânica, c.p. 355, CEP 14800-900, Araraquara, SP, Brazil.
Nat Prod Commun. 2010 Aug;5(8):1225-32.
Phenolic compounds are produced by secretory idioblasts and hypodermis, and by specialized cells of the epidermis and chlorenchyma of leaves of Alchornea triplinervia. Phytochemical investigation of these leaves led to the isolation of the known substances quercetin, quercetin-7-O-beta-D-glucopyranoside, quercetin-3-O-beta-D-glucopyranoside, quercetin-3-O-beta-D-galactopyranoside, quercetin-3-O-alpha-L-arabinopyranoside, amentoflavone, brevifolin carboxylic acid, gallic acid, and methyl gallate from the methanolic extract, and stigmasterol, campesterol, sitosterol, lupeol, friedelan-3-ol, and friedelan-3-one from the chloroform extract. In studies of antibacterial activity and mutagenicity, the methanolic extract showed promising activity against Staphylococcus aureus (MIC = 62.5 microg/mL) and was slightly mutagenic in vitro and in vivo at the highest concentrations tested (1335 mg/kg b.w.).
酚类化合物由三脉叶下珠的分泌异细胞和皮下组织，以及叶片表皮和叶肉的特化细胞产生。对这些叶片进行植物化学研究，从甲醇提取物中分离出了已知物质槲皮素、槲皮素 - 7 - O - β - D - 吡喃葡萄糖苷、槲皮素 - 3 - O - β - D - 吡喃葡萄糖苷、槲皮素 - 3 - O - β - D - 吡喃半乳糖苷、槲皮素 - 3 - O - α - L - 吡喃阿拉伯糖苷、穗花杉双黄酮、 brevifolin 羧酸、没食子酸和没食子酸甲酯，从氯仿提取物中分离出了豆甾醇、菜油甾醇、β - 谷甾醇、羽扇豆醇、木栓烷 - 3 - 醇和木栓烷 - 3 - 酮。在抗菌活性和致突变性研究中，甲醇提取物对金黄色葡萄球菌显示出有前景的活性（最低抑菌浓度 = 62.5微克/毫升），并且在最高测试浓度（1335毫克/千克体重）下在体外和体内有轻微的致突变性。
