Internal Medicine Postgraduate Program, Universidade Federal do Paraná, Curitiba, Brazil.
J Clin Pathol. 2010 Oct;63(10):930-4. doi: 10.1136/jcp.2010.077867.
BACKGROUND/AIM: Acute respiratory infections are an important cause of childhood morbidity and mortality throughout the world, and viruses have often been reported to be an aetiological agent. This study aimed to identify respiratory viruses in paraffin-embedded samples of paediatric lung necropsy specimens, using immunohistochemistry on tissue microarray slides.
Retrospective study in 200 lung tissue samples from children who had died from severe respiratory infections during 1985-2005. Immunoperoxidase assay was performed to detect the viruses that were most commonly associated with respiratory tract infections: influenza virus A (FLU A), influenza virus B (FLU B), respiratory syncytial virus (RSV), adenovirus (AdV) and parainfluenza virus (PIV) types 1, 2 and 3.
Viruses were detected in 71 (35.5%) cases. Most positive cases were observed in children younger than 6 months. In 42.3% of cases, only one virus was detected: 11 (36.7%) RSV; 7 (23.3%) AdV; 4 (13.3%) PIV2; 3 (10%) FLU A; 2 (6.7%) FLU B; 2 (6.7%) PIV3; and 1 (3.3%) PIV1. Co-infection with more than one virus was observed in 41 (57.7%) cases. No positive correlations were observed between the presence of viral antigens and seasonality of the infection, sex, age or histopathological findings.
Non-pandemic seasonal respiratory viruses are involved in a significant number of deaths in paediatric patients; these findings highlight the importance of laboratory investigation of these agents in patients hospitalised with severe acute respiratory infections.
背景/目的：急性呼吸道感染是全世界儿童发病率和死亡率的重要原因，病毒通常被报道为病因。本研究旨在使用组织微阵列载玻片上的免疫组织化学方法，鉴定石蜡包埋的儿科肺尸检标本中的呼吸道病毒。
对 1985 年至 2005 年间死于严重呼吸道感染的 200 例儿童肺组织样本进行回顾性研究。免疫过氧化物酶检测法用于检测与呼吸道感染最相关的病毒：甲型流感病毒（FLU A）、乙型流感病毒（FLU B）、呼吸道合胞病毒（RSV）、腺病毒（AdV）和副流感病毒（PIV）1、2 和 3 型。
在 71 例（35.5%）病例中检测到病毒。大多数阳性病例发生在 6 个月以下的儿童中。在 42.3%的病例中，仅检测到一种病毒：11 例（36.7%）为 RSV；7 例（23.3%）为 AdV；4 例（13.3%）为 PIV2；3 例（10%）为 FLU A；2 例（6.7%）为 FLU B；2 例（6.7%）为 PIV3；1 例（3.3%）为 PIV1。41 例（57.7%）病例中观察到混合感染。病毒抗原的存在与感染的季节性、性别、年龄或组织病理学发现之间无明显相关性。
非大流行季节性呼吸道病毒在儿科患者的大量死亡中起重要作用；这些发现强调了对住院严重急性呼吸道感染患者进行这些病原体实验室检测的重要性。
