大肠杆菌 CRISPR 系统可抵御 λ 溶原菌、溶原菌和噬菌体诱导。
The Escherichia coli CRISPR system protects from λ lysogenization, lysogens, and prophage induction.
机构信息
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
出版信息
J Bacteriol. 2010 Dec;192(23):6291-4. doi: 10.1128/JB.00644-10. Epub 2010 Oct 1.
Abstract
We show that phage lysogenization, lysogens, and prophage induction are all targeted by CRISPR. The results demonstrate that genomic DNA is not immune to the CRISPR system, that the CRISPR system does not require noncytoplasmic elements, and that the system protects from phages entering and exiting the lysogenic cycle.
摘要
我们证明了噬菌体溶原化、溶原菌和前噬菌体诱导都受到 CRISPR 的靶向作用。结果表明，基因组 DNA 并非不受 CRISPR 系统的影响，该系统不需要非细胞质元件，并且该系统可以防止噬菌体进出溶原周期。
相似文献
1
The Escherichia coli CRISPR system protects from λ lysogenization, lysogens, and prophage induction.
J Bacteriol. 2010 Dec;192(23):6291-4. doi: 10.1128/JB.00644-10. Epub 2010 Oct 1.
2
Bacteriophage lambda: a paradigm revisited.
J Virol. 2010 Jul;84(13):6876-9. doi: 10.1128/JVI.02177-09. Epub 2010 Apr 7.
3
Carriage of λ Latent Virus Is Costly for Its Bacterial Host due to Frequent Reactivation in Monoxenic Mouse Intestine.
PLoS Genet. 2016 Feb 12;12(2):e1005861. doi: 10.1371/journal.pgen.1005861. eCollection 2016 Feb.
4
Lysis-lysogeny coexistence: prophage integration during lytic development.
Microbiologyopen. 2017 Feb;6(1). doi: 10.1002/mbo3.395. Epub 2016 Aug 17.
5
Roles of and in Development of Bacteriophages λ and Φ24.
Viruses. 2018 Oct 11;10(10):553. doi: 10.3390/v10100553.
6
Mechanisms that Determine the Differential Stability of Stx⁺ and Stx(-) Lysogens.
Toxins (Basel). 2016 Mar 31;8(4):96. doi: 10.3390/toxins8040096.
7
Introduction to lambda phages.
Curr Protoc Mol Biol. 2001 May;Chapter 1:Unit1.9. doi: 10.1002/0471142727.mb0109s13.
8
The book of Lambda does not tell us that naturally occurring lysogens of are likely to be resistant as well as immune.
Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2212121120. doi: 10.1073/pnas.2212121120. Epub 2023 Mar 7.
9
Elements in the λ immunity region regulate phage development: beyond the 'Genetic Switch'.
Mol Microbiol. 2019 Dec;112(6):1798-1813. doi: 10.1111/mmi.14394. Epub 2019 Oct 8.
10
E. coli K12 inf: a mutant deficient in prophage lambda induction and cell filamentation.
Mol Gen Genet. 1975;136(4):291-307. doi: 10.1007/BF00341714.
引用本文的文献
1
CRISPR-Cas Systems: A Functional Perspective and Innovations.
Int J Mol Sci. 2025 Apr 12;26(8):3645. doi: 10.3390/ijms26083645.
2
Gut phages and their interactions with bacterial and mammalian hosts.
J Bacteriol. 2025 Feb 20;207(2):e0042824. doi: 10.1128/jb.00428-24. Epub 2025 Jan 23.
3
Nucleic acid recognition during prokaryotic immunity.
Mol Cell. 2025 Jan 16;85(2):309-322. doi: 10.1016/j.molcel.2024.12.007.
4
From resistance to remedy: the role of clustered regularly interspaced short palindromic repeats system in combating antimicrobial resistance-a review.
Naunyn Schmiedebergs Arch Pharmacol. 2025 Mar;398(3):2259-2273. doi: 10.1007/s00210-024-03509-6. Epub 2024 Oct 15.
5
Escherichia coli CRISPR arrays from early life fecal samples preferentially target prophages.
ISME J. 2024 Jan 8;18(1). doi: 10.1093/ismejo/wrae005.
6
synthesizes an R-type bacteriocin.
Appl Environ Microbiol. 2024 Jan 24;90(1):e0127323. doi: 10.1128/aem.01273-23. Epub 2024 Jan 3.
7
Analysis of CRISPR/Cas Genetic Structure, Spacer Content and Molecular Epidemiology in Brazilian Clinical Isolates.
Pathogens. 2023 May 26;12(6):764. doi: 10.3390/pathogens12060764.
8
A DNA phosphorothioation-based Dnd defense system provides resistance against various phages and is compatible with the Ssp defense system.
mBio. 2023 Aug 31;14(4):e0093323. doi: 10.1128/mbio.00933-23. Epub 2023 Jun 1.
9
Engineered phage with antibacterial CRISPR-Cas selectively reduce E. coli burden in mice.
Nat Biotechnol. 2024 Feb;42(2):265-274. doi: 10.1038/s41587-023-01759-y. Epub 2023 May 4.
10
CRISPR-Cas Controls Cryptic Prophages.
Int J Mol Sci. 2022 Dec 19;23(24):16195. doi: 10.3390/ijms232416195.
本文引用的文献
1
Interference with histidyl-tRNA synthetase by a CRISPR spacer sequence as a factor in the evolution of Pelobacter carbinolicus.
BMC Evol Biol. 2010 Jul 28;10:230. doi: 10.1186/1471-2148-10-230.
2
Transcription, processing and function of CRISPR cassettes in Escherichia coli.
Mol Microbiol. 2010 Sep;77(6):1367-79. doi: 10.1111/j.1365-2958.2010.07265.x.
3
Self-targeting by CRISPR: gene regulation or autoimmunity?
Trends Genet. 2010 Aug;26(8):335-40. doi: 10.1016/j.tig.2010.05.008. Epub 2010 Jul 1.
4
Bacteriophage resistance mechanisms.
Nat Rev Microbiol. 2010 May;8(5):317-27. doi: 10.1038/nrmicro2315. Epub 2010 Mar 29.
5
Identification and characterization of E. coli CRISPR-cas promoters and their silencing by H-NS.
Mol Microbiol. 2010 Mar;75(6):1495-512. doi: 10.1111/j.1365-2958.2010.07073.x. Epub 2010 Feb 1.
6
CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea.
Nat Rev Genet. 2010 Mar;11(3):181-90. doi: 10.1038/nrg2749.
7
Self versus non-self discrimination during CRISPR RNA-directed immunity.
Nature. 2010 Jan 28;463(7280):568-71. doi: 10.1038/nature08703. Epub 2010 Jan 13.
8
CRISPR/Cas, the immune system of bacteria and archaea.
Science. 2010 Jan 8;327(5962):167-70. doi: 10.1126/science.1179555.
9
RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex.
Cell. 2009 Nov 25;139(5):945-56. doi: 10.1016/j.cell.2009.07.040.
10
CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA.
Science. 2008 Dec 19;322(5909):1843-5. doi: 10.1126/science.1165771.
Zotero 插件