Centre for Epidemiology and Biostatistics, Leeds Institute of Genetics, Health and Therapeutics University of Leeds, Leeds, UK.
Food Chem Toxicol. 2011 Jan;49(1):132-5. doi: 10.1016/j.fct.2010.10.007. Epub 2010 Oct 8.
Deoxynivalenol (DON) requires no activation for toxicity, though susceptibility may reflect individual variations in detoxification. This study reports the measurement of un-metabolised urinary DON (free DON) and DOM-1 in samples previously analysed for the combined measure of free DON+DON-glucuronide (fD+DG), with a concentration >5 ng/ml, for 34 UK adults. Four consecutive daily urine samples were analysed from twenty-two individuals, whilst from 12 individuals only a single sample was analysed. The mean (median) concentration of urinary fD+DG in this sub-set was 17.8 ng/ml (13.8 ng/ml), range 5.0-78.2 ng/ml. In 23/34 (68%) individuals, free DON was detected, mean 2.4 ng/ml; range 0.5-9.3 ng/ml. Urinary DOM-1 was detected in 1/34 (3%) of individuals; present at ∼1% of urinary fD+DG concentration for that individual. The concentration of fD+DG combined was significantly correlated with urinary free DON (p<0.001, R(2)=0.65), but not with the percentage of free DON to fD+DG (p=0.615, R(2)=0.01), suggesting that the level of DON exposure did not affect the metabolism to DG within the range observed. In this survey most individuals had no detectable urinary DOM-1 and 68% did not detoxify all of the ingested DON to DON-glucuronide. This study needs to be extended to understand whether the fD / DG ratio provides a phenotypic measure of DON susceptibility.
脱氧雪腐镰刀菌烯醇（DON）不需要激活即可产生毒性，尽管易感性可能反映了个体解毒能力的差异。本研究报告了对先前分析过的组合游离 DON+DON-葡萄糖醛酸（fD+DG）浓度>5ng/ml 的 34 名英国成年人尿液中未代谢的游离 DON（游离 DON）和 DOM-1 的测量值，其中 22 名个体连续 4 天分析了 4 次尿液样本，而 12 名个体仅分析了 1 次尿液样本。该亚组尿液中 fD+DG 的平均（中位数）浓度为 17.8ng/ml（13.8ng/ml），范围为 5.0-78.2ng/ml。在 34 名个体中的 23 名（68%）中检测到游离 DON，平均浓度为 2.4ng/ml，范围为 0.5-9.3ng/ml。在 34 名个体中的 1 名（3%）中检测到 DOM-1，存在于该个体尿液 fD+DG 浓度的 1%左右。fD+DG 联合浓度与尿液游离 DON 显著相关（p<0.001，R²=0.65），但与游离 DON 占 fD+DG 的百分比无关（p=0.615，R²=0.01），表明在观察到的范围内 DON 暴露水平不会影响 DG 的代谢。在本研究中，大多数个体尿液中未检测到 DOM-1，68%的个体未将摄入的 DON 全部代谢为 DON-葡萄糖醛酸。本研究需要进一步扩展，以了解 fD / DG 比值是否提供了 DON 易感性的表型测量。
