合成的核糖开关可在多种细菌物种中诱导基因表达。
Synthetic riboswitches that induce gene expression in diverse bacterial species.
机构信息
Department of Chemistry, Center for Fundamental and Applied Molecular Evolution, Emory University, Atlanta, GA 30322, USA.
出版信息
Appl Environ Microbiol. 2010 Dec;76(23):7881-4. doi: 10.1128/AEM.01537-10. Epub 2010 Oct 8.
Abstract
We developed a series of ligand-inducible riboswitches that control gene expression in diverse species of Gram-negative and Gram-positive bacteria, including human pathogens that have few or no previously reported inducible expression systems. We anticipate that these riboswitches will be useful tools for genetic studies in a wide range of bacteria.
摘要
我们开发了一系列配体诱导的核糖开关，可在革兰氏阴性和革兰氏阳性细菌的多种物种中控制基因表达，包括那些先前报道的诱导表达系统很少或没有的人类病原体。我们预计这些核糖开关将成为广泛应用于各种细菌的遗传研究的有用工具。
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