College of Nanoscale Science and Engineering, University at Albany, SUNY, Albany, NY, USA.
Integr Biol (Camb). 2010 Nov;2(11-12):696-706. doi: 10.1039/c0ib00044b. Epub 2010 Oct 12.
This study presents the design and optimization for in vitro use of a new versatile chemotaxis device called the NANIVID (NANo IntraVital Imaging Device), developed using advanced nano/micro fabrication techniques. The device is fabricated using microphotolithographic techniques and two substrates are bonded together using a thin polymer layer creating a sealed device with one outlet. The main structure of the device consists of two Pyrex substrates: an etched chemoattractant reservoir and a top cover, with a final size of 0.2 × 2 × 3 mm. This reservoir contains a hydrogel blend with EGF which diffuses out through a small (∼9.10(3)μm(2)) outlet. This reservoir sustains a steady release of growth factor into the surrounding environment for several hours establishing a consistent concentration gradient from the device. The focus of this study was to design and optimize the new device for cell chemotaxis studies in breast cancer cells in cell culture. Our results show that we have created a flexible, cheap, miniature and autonomous chemotaxis device and demonstrate its usefulness in 2D and 3D cell culture. We also provide preliminary data for use of the device in vivo.
本研究提出了一种新的多功能趋化性装置（称为 NANIVID，即纳米活体成像装置）的设计和优化，该装置使用先进的纳米/微制造技术开发而成。该装置采用微光刻技术制造，两个基板通过薄聚合物层粘合在一起，形成一个带有一个出口的密封装置。该装置的主要结构由两个 Pyrex 基板组成：一个蚀刻的趋化剂储液器和一个顶盖，最终尺寸为 0.2×2×3mm。该储液器含有 EGF 水凝胶混合物，通过一个小的（∼9.10(3)μm(2)）出口扩散出来。该储液器能够持续数小时向周围环境中稳定释放生长因子，从而在装置内建立一致的浓度梯度。本研究的重点是设计和优化新装置，以用于乳腺癌细胞在细胞培养中的细胞趋化性研究。我们的结果表明，我们已经创建了一种灵活、廉价、微型和自主的趋化性装置，并证明了其在 2D 和 3D 细胞培养中的有用性。我们还提供了该装置在体内使用的初步数据。
