Lee Jong Min, Seo Hye In, Bae Jun Hyuk, Chung Bong Geun
Department of Mechanical Engineering, Sogang University, Seoul, Korea.
Electrophoresis. 2017 May;38(9-10):1318-1324. doi: 10.1002/elps.201600540. Epub 2017 Mar 10.
We developed the photo-crosslinkable hydrogel microfluidic co-culture device to study photothermal therapy and cancer cell migration. To culture MCF7 human breast carcinoma cells and metastatic U87MG human glioblastoma in the microfluidic device, we used 10 w/v% gelatin methacrylate (GelMA) hydrogels as a semi-permeable physical barrier. We demonstrated the effect of gold nanorod on photothermal therapy of cancer cells in the microfluidic co-culture device. Interestingly, we observed that metastatic U87MG human glioblastoma largely migrated toward vascular endothelial growth factor (VEGF)-treated GelMA hydrogel-embedding microchannels. The main advantage of this hydrogel microfluidic co-culture device is to simultaneously analyze the physiological migration behaviors of two cancer cells with different physiochemical motilities and study gold nanorod-mediated photothermal therapy effect. Therefore, this hydrogel microfluidic co-culture device could be a potentially powerful tool for photothermal therapy and cancer cell migration applications.
我们开发了可光交联水凝胶微流控共培养装置，用于研究光热疗法和癌细胞迁移。为了在微流控装置中培养MCF7人乳腺癌细胞和转移性U87MG人胶质母细胞瘤细胞，我们使用10 w/v%的甲基丙烯酸明胶（GelMA）水凝胶作为半透性物理屏障。我们展示了金纳米棒在微流控共培养装置中对癌细胞光热疗法的效果。有趣的是，我们观察到转移性U87MG人胶质母细胞瘤大量迁移至经血管内皮生长因子（VEGF）处理的嵌入GelMA水凝胶的微通道。这种水凝胶微流控共培养装置的主要优势在于能够同时分析两种具有不同物理化学运动性的癌细胞的生理迁移行为，并研究金纳米棒介导的光热疗法效果。因此，这种水凝胶微流控共培养装置可能是用于光热疗法和癌细胞迁移应用的一种潜在强大工具。
