Departments of Surgery, University of California, 9500 Gilman Drive, San Diego, California, 92093-0068, USA.
Infect Agent Cancer. 2010 Oct 12;5:17. doi: 10.1186/1750-9378-5-17.
Infection with human papillomavirus (HPV) is a major risk factor for development of anal squamous cell carcinoma. Despite over 100 genotypes of the virus, HPV 16 and 18 are considered pathogenic as they are seen in the majority of cervical and anal cancers. We have employed a custom microarray to examine DNA for several HPV genotypes. We aimed to determine the accuracy of our microarray in anal cancer DNA for HPV genotypes compared to the DNA sequencing gold standard.
We utilized a sensitive microarray platform to classify 37 types of mucosal HPVs including 14 known high-risk and 23 low-risk types based on cervical cancer data. We utilized DNA from pathologically confirmed cases of anal squamous cell carcinoma. All samples underwent microarray HPV genotyping and PCR analysis.
HPV was detected in 18/20 (90%) anal cancers. HPV genotypes 16 and 18 were present in the majority of specimens, with HPV 16 being the most common. Eighty percent of anal cancers had at least two HPV types. Ten percent of cases (2/20) tested negative using our microarray; DNA sequencing confirmed the lack of presence of HPV DNA in these samples.
Microarray technology is an accurate way to screen for various genotypes of HPV in anal cancer, with 100% correlation with genomic DNA detection of HPV. The majority of anal cancers in our study associated with pathogenic HPV 16 and/or 18. Other HPV genotypes are present simultaneously with HPV 16 and 18, and might contribute to its pathogenesis.
人乳头瘤病毒（HPV）感染是肛门鳞状细胞癌发展的主要危险因素。尽管病毒有 100 多种基因型，但 HPV 16 和 18 被认为是致病的，因为它们存在于大多数宫颈癌和肛门癌中。我们使用了定制的微阵列来检查几种 HPV 基因型的 DNA。我们旨在确定我们的微阵列在肛门癌 DNA 中用于 HPV 基因型的准确性，与 DNA 测序金标准相比。
我们利用一种敏感的微阵列平台，根据宫颈癌数据对 37 种黏膜 HPV 进行分类，包括 14 种已知的高危型和 23 种低危型。我们利用经病理证实的肛门鳞状细胞癌的 DNA。所有样本均进行微阵列 HPV 基因分型和 PCR 分析。
18/20（90%）例肛门癌中检测到 HPV。HPV 16 和 18 型存在于大多数标本中，其中 HPV 16 最为常见。80%的肛门癌有至少两种 HPV 类型。10%的病例（2/20）使用我们的微阵列检测为阴性；DNA 测序证实这些样本中不存在 HPV DNA。
微阵列技术是一种在肛门癌中筛查各种 HPV 基因型的准确方法，与 HPV 基因组 DNA 检测具有 100%的相关性。我们研究中的大多数肛门癌与致病性 HPV 16 和/或 18 相关。其他 HPV 基因型与 HPV 16 和 18 同时存在，可能有助于其发病机制。
