Delori F C
Appl Opt. 1994 Nov 1;33(31):7439-52. doi: 10.1364/AO.33.007439.
A spectrophotometer for noninvasively measuring the intrinsic fluorescence and the reflectance of the ocular fundus is described. The instrument uses multichannel spectral analysis for recording fluorescence emission spectra (500-800 nm) with seven excitation wavelengths between 430 and 550 nm and for the determination of fundus reflectance spectra (400-800 nm). Measurements are performed from a discrete fundus area, with a spatial resolution of a 1-2° visual angle. Calibration procedures are detailed. Representative fluorescence and reflectance spectra obtained from five normal subjects indicate that the fluorescence originates from within the fundus layers. Although the absolute fundus fluorescence measurement is affected by lens absorption and ocular refraction, it is minimally influenced by the strong fluorescence of the crystalline lens.
本文描述了一种用于非侵入性测量眼底固有荧光和反射率的分光光度计。该仪器使用多通道光谱分析来记录荧光发射光谱（500 - 800nm），激发波长在430至550nm之间，共七个波长，同时用于测定眼底反射光谱（400 - 800nm）。测量是从离散的眼底区域进行的，空间分辨率为1 - 2°视角。详细介绍了校准程序。从五名正常受试者获得的代表性荧光和反射光谱表明，荧光源自眼底各层内部。尽管绝对眼底荧光测量受晶状体吸收和眼屈光的影响，但晶状体的强荧光对其影响最小。
